高效根结线虫生防真菌筛选及其性能研究  

Screening and performance of high-efficiency root-knot nematode biocontrol fungi

作　　者：李磊 赵俊杰[1,2] 刘莹莹[2] 甄静[1,2] 李亮亮[1,2] 陈国参 慕琦[2] 王继雯[1,2] LI Lei;ZHAO Junjie;LIU Yingying;ZHEN Jing;LI Liangliang;CHEN Guocan;MU Qi;WANG Jiwen(Henan Provincial Key Laboratory of Microbial Engineering,Zhengzhou 450000,China;Henan Academy of Sciences Institute of Biology Co.,Ltd,Zhengzhou 450000,China)

机构地区：[1]河南省微生物工程重点实验室,郑州450000 [2]河南省科学院生物研究所有限责任公司,郑州450000

出　　处：《生物学杂志》2021年第6期70-74,81,共6页Journal of Biology

基　　金：河南省科学院科技开发补助项目(190305004);河南省科技攻关项目(182102110036);河南省科学院科技开发补助项目(200305002)。

摘　　要：为了获得具有防治根结线虫作用的菌株,采用几丁质平板透明圈初筛和离体拮抗试验复筛的方法,得到一株具有生防潜力的真菌菌株SFC-3。通过形态学特征观察和真菌内转录间隔区序列(Internal transcribed spacer,ITS)分析鉴定,确定菌株SFC-3为棘孢木霉(Trichoderma asperellum),其几丁质酶活为2.07 U/mL。根结线虫虫卵寄生实验和二龄幼虫致死率实验表明:菌株SFC-3对虫卵的寄生率最高达到78.86%,高于SFC-1、SFC-22等其他9株菌,在第7天时对根结线虫虫卵孵化的相对抑制率最高达到96.51%,对二龄幼虫的校正死亡率达到93.37%。通过筛选抗根结线虫菌株并研究了其生防效果,证实了菌株SFC-3有比较优秀的抗根结线虫性能。In order to obtain strains with the effect of preventing and controlling root-knot nematodes,the chitin flat transparent ring primary screening and the in vitro antagonistic test rescreening methods were used to obtain a fungal strain SFC-3 with biocontrol potential.Through observation of morphological characteristics and analysis and identification of transcribed spacer sequences(ITS)in fungi,the strain SFC-3 was identified as Trichoderma asperellum and its chitinase activity was 2.07 U/mL.The root-knot nematode egg parasitic experiment and the second-instar larval lethality experiment showed that the strain SFC-3 had the highest parasitic rate of 78.86%,which was higher than SFC-1,SFC-22 and other 9 strains.At the 7th day,the relative inhibition rate of egg incubation of root-knot nematodes reached 96.51%,and the corrected mortality rate of second-instar larvae reached 93.37%.In this study,by screening anti-knot nematode strains and studying their biocontrol effects,it was confirmed that the strain SFC-3 had better anti-root knot nematode performance.

关 键 词：根结线虫 棘孢木霉 几丁质酶活 种质资源 生物防治 

分 类 号：Q939.9[生物学—微生物学] S435[农业科学—农业昆虫与害虫防治]