衢枳壳总黄酮通过NF-κB信号通路对RSV感染哮喘小鼠肺损伤的保护作用  

Protective effect of total flavonoids from Fructus aurantii on lung injury of asthma mice infected with RSV through NF-κB signaling pathway

作　　者：刘小娟 姜小琴[2] 方月娟 夏道宗[3] 王思为[4] 钟松阳[5] LIU Xiao-juan;JIANG Xiao-qin;FANG Yue-juan;XIA Dao-zong;WANG Si-wei;ZHONG Song-yang(Quzhou Maternal and Child Health Hospital,Quzhou,Zhejiang 324000,China;不详)

机构地区：[1]衢州市妇幼保健院药剂科,浙江衢州324000 [2]江山市人民医院药剂科,浙江衢州324000 [3]浙江中医药大学研究生院,浙江杭州310053 [4]衢州市人民医院中心实验室,浙江衢州324000 [5]衢州市人民医院药剂科,浙江衢州324000

出　　处：《中华医院感染学杂志》2021年第22期3376-3380,共5页Chinese Journal of Nosocomiology

基　　金：衢州市科技计划重点基金资助项目(2016J016);衢州市科技局指导性基金资助项目(2016114)。

摘　　要：目的探讨衢枳壳总黄酮通过核因子-κB(NF-κB)信号通路对呼吸道合胞病毒(RSV)感染哮喘小鼠肺损伤的保护作用。方法雌性BALB/c小鼠36只,随机分成正常组、模型组、阳性组和衢枳壳总黄酮低、中、高剂量组,每组6只。采用卵清蛋白(VOA)、RSV液建立哮喘模型;观察气道反应性;酶联免疫吸附法检测血清中白细胞介素(IL)-4、IL-5、IL-13表达;生化法检测肺组织超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH-Px),肺组织进行苏木精-伊红(HE)染色,蛋白免疫印迹法(WB)检测NF-κB P65、核因子抑制蛋白(IκB)、IκB激酶(IκK)及半胱氨酸天冬氨酸蛋白-3(Caspase-3)蛋白表达。结果与模型组比,衢枳壳总黄酮不同剂量组肺组织SOD、GSH-Px水平均升高,MDA水平降低,小鼠血清IL-4、IL-5及IL-13表达水平均降低(均P<0.05)。衢枳壳总黄酮不同剂量组及阳性组Caspase-3及细胞质IκBα、NF-κB P65蛋白表达均升高(均P<0.05)。结论衢枳壳总黄酮对RSV感染哮喘小鼠有抗炎、抗氧化应激作用,机制可能与NF-κB信号通路有关。OBJECTIVE To explore the protective effect of total flavonoids from Quzhiqiao((Fructus Aurantii)) on lung injury of asthma mice infected with respiratory syncytial virus(RSV) through nuclear factor-κB(NF-κB) signaling pathway. METHODS Totally 36 female BALB/c mice were randomly divided into the normal group, the model group, the positive group, the high-dose total flavonoids of Quzhiqiao group, the medium-dose total flavonoids of Quzhiqiao group and the low-dose total flavonoids of Quzhiqiao group, with 6 mice in each group. The asthma models were established by ovalbumin(VOA) and RSV solution. The reactivity of airway was observed, the expression levels of serum interleukin(IL)-4, IL-5 and IL-13 were detected with the use of enzyme-linked immunosorbent assay, the superoxide dismutase(SOD), malondialdehyde(MDA) and glutathione peroxidase(GSH-Px) in lung tissues were detected by biochemical method, Hematoxylin-eosin(HE) staining was performed for lung tissues, and the expression levels of NF-κB P65, nuclear factor inhibitor protein(IκB), IκB kinase(IκK) and caspase-3(Caspase-3) were detected by using western blot(WB). RESULTS The levels of SOD and GSH-Px in lung tissues of the three total flavonoids of Quzhiqiao groups were significantly lower than those of the model group, the MDA level was reduced, significantly the expression levels of serum IL-4, IL-5 and IL-13 of the mice were reduced significantly(all P<0.05).The expression levels of Caspase-3 and cytoplasmic IκBα, NF-κB P65 were significantly elevated in the three total flavonoids of Quzhiqiao groups and the positive groups(all P<0.05). CONCLUSION Total flavonoids from Fructus aurantii has anti-inflammatory and anti-oxidative stress effect on the asthma mice with RSV infection, and the mechanisms may be related to the NF-κB signaling pathway.

关 键 词：衢枳壳总黄酮 支气管哮喘 核转录因子-ΚB 氧化应激 小鼠 

分 类 号：R562.25[医药卫生—呼吸系统]