全自动染色体收获仪在淋巴细胞微核试验中的价值  

The value of automatic chromosome harvester in lymphocyte micronucleus test

作　　者：钱小莲 方慧莲 QIAN Xiaolian;FANG Huilian(Nanjing Prevention and Treatment Center for Occupational Diseases,Nanjing 210042 China)

机构地区：[1]南京市职业病防治院检验科,江苏南京210042

出　　处：《中国辐射卫生》2021年第5期560-563,共4页Chinese Journal of Radiological Health

摘　　要：目的探讨全自动染色体收获仪在淋巴细胞微核试验中的优势。方法采集80名放射工作人员的外周血,分别用全自动染色体收获仪和手工法收获淋巴细胞,吉姆萨染片后,在显微镜下观察淋巴细胞的微核。结果对同一批标本收获淋巴细胞,全自动收获仪用时(185 min)小于人工组(350 min),使用收获仪得到的微核率(1.04‰±0.63‰)、微核细胞率(0.89‰±0.69‰)和微核阳性率(70.0%)均高于手工法(0.60‰±0.68‰,0.51‰±0.55‰,48.8%),差异均有统计学意义(P <0.01)。结论全自动染色体收获仪可以缩短实验操作的时间,减轻实验室人员的工作量,保护实验人员的身心健康;同时提高淋巴细胞微核的检出率,为临床提供更为准确的检测数据。Objective To explore the advantages of automatic chromosome harvester in lymphocyte micronucleus test.Methods The peripheral blood of 80 radiation workers was collected, then lymphocytes were harvested with an automatic chromosome harvester and manual methods. After Giemsa stained the slides, the micronucleus of the lymphocytes was analyzed under a microscope. Results For lymphocytes from the same batch of specimens, the time of the automatic harvester(185 min) was less than that of the artificial group(350 min). The micronucleus rate(1.04‰ ± 0.63‰), micronucleus cell rate(0.89‰ ± 0.69) and micronucleus positive rate(70.0%) that obtained by the harvester were all higher than manual method(0.60‰ ± 0.68‰, 0.51‰ ± 0.55‰, 48.8%), and the difference was statistically significant(P < 0.01). Conclusion The automatic chromosome harvester can shorten the experimental operation time, reduce the workload of laboratory personnel,and protect the physical and mental health of laboratory personnel. At the same time, it can increase the detection rate of lymphocyte micronucleus, which can provide more accurate detection data for the clinic.

关 键 词：全自动染色体收获仪 微核率 微核细胞率 微核阳性率 

分 类 号：X591[环境科学与工程—环境工程]