载脱钙骨基质的3D打印多孔生物陶瓷的制备及其成骨性能研究  

Preparation of DBM-loaded 3D-printed porous bioceramics and osteogenesis properties

作　　者：张铁 胡丽 蔡志祥 杜小青 闫飞飞[3] 王志勇 张旗 蔡林[3] Zhang Tie;Hu Li;Cai Zhixiang;Du Xiaoqing;Yan Feifei;Wang Zhiyong;Zhang Qi;Cai Lin(Hubei Osteolink Biomaterial Co.,Ltd.(Wuhan Hi-tech Research Center of Medical Tissues),Wuhan Hubei,430000;Wuhan Intelligent Laser Technology Co.,Ltd,Wuhan Hubei,430000;Zhongnan Hospital of Wuhan University,Wuhan Hubei,430071,China)

机构地区：[1]湖北联结生物材料有限公司(武汉高科医用组织研究中心),湖北武汉430000 [2]武汉因泰莱激光科技有限公司,湖北武汉430000 [3]武汉大学中南医院骨科,湖北武汉430071

出　　处：《生物骨科材料与临床研究》2021年第6期1-6,共6页Orthopaedic Biomechanics Materials and Clinical Study

基　　金：武汉市年度前资助科技计划项目(2020020602012098)。

摘　　要：目的以光固化成型技术制备高精度3D打印多孔生物陶瓷,同时在多孔生物陶瓷的孔道中引入脱钙骨基质(DBM),研究其生物相容性和成骨能力,探讨3D打印多孔生物陶瓷用于临床的可能性。方法以固相法合成高纯度β-磷酸三钙,通过光固化成型技术打印以β-磷酸三钙为基材的高精度模型,经高温烧结除去光固化树脂等有机成分,得到3D打印多孔生物陶瓷(3D-TCP),将DBM引入3D打印多孔生物陶瓷的孔道中,得到成骨能力更佳的3D打印多孔生物陶瓷(3D-TCP/DBM);观察β-磷酸三钙的晶型和粒径,以及研究烧结温度对多孔生物陶瓷力学性能和微观形态的影响,并考察其生物相容性和体内成骨能力。结果固相法获得β-磷酸三钙粒径(d90)为(3.42±1.42) m,晶型结构特征符合ICDD的PDF卡09-0169,无明显杂质峰,在1 250℃下烧结的样品力学强度最佳,达到(19.35±2.94) MPa,微观结构均匀致密,3D-TCP无细胞毒性,显示良好的生物相容性,8周切片显示3D-TCP孔隙中有大量新生骨组织,3D-TCP/DBM中DBM转化为新骨,并可见周围孔隙存在新生骨组织。结论 3D-TCP具有良好的生物相容性,在其孔道中引入DBM后具有更佳的成骨能力,有望用于临床。Objective High precision 3 D printed porous bioceramics were prepared by light curing technology, and decalcified bone matrix(DBM) at the same time was introduced into the pores of porous bioceramics to study its biocompatibility and osteogenic ability, and to explore the possibility of 3 D printed porous bioceramics for clinical application. Methods High-purity β-tricalcium phosphate was synthesized by solid phase method, 3 D-printed scaffolds were prepared by SLA curing technology with β-tricalcium phosphate as the base material, the scaffolds were degreased and sintered to remove the organic components, and the 3 D-printed porous bioceramics(3 D-TCP) were prepared. Then DBM was added into the channel of 3 D-printed porous bioceramics to obtain an DBM-loaded 3 D-printed porous bio ceramics(3 D-TCP/DBM) with better osteogenic properties. The crystal shape and particle size of β-tricalcium p hosphate were observed,and the effect of sintering temperature on the mechanical properties and microscopic morphology of porous bioceramics was studied, and the biocompatibility and osteogenic properties of the porous bioceramics were investigated. Results The particle size(d90) of β-tricalcium phosphate was(3.42±1.42) m, and a typical β-tricalcium phosphate XRD pattern showed the peaks from ICDD # 09-0169, there was no obvious impurity peak. The mechanical strength of the sample sintered at 1 250℃ reached(19.35±2.94) MPa, the microstructure was dense and homogenous.3 D-TCP showed no cytotoxicity and good biocompatibility. The results of 8-week sectioning showed a large amount of new bone tissue in the 3 D-TCP pore, and the DBM was transformed into new bone in the 3 D-TCP/DBM. Conclusion3 D-TCP has good biocompatibility, the addition of DBM in the channel has better osteogenic properties, which is expected to be used in clinical application.

关 键 词：光固化 Β-磷酸三钙 脱钙骨基质 多孔 成骨 

分 类 号：R318.08[医药卫生—生物医学工程]