硝基还原酶制备及检测  

Preparationand Detection of Nitroreductase

作　　者：安亚萱 韩蕊 王凯悦 王玉彬 毛淑红[2] 刘凯[1] 朱柏林 AN Ya-xuan;HAN Rui;WANG Kai-yue;WANG Yu-bin;MAO Shu-hong;LIU Kai;ZHU Bo-lin(College of Chemistry,Tianjin Normal University,Tianjin 300387;College of Biotechnology,Tianjin University of Science and Technology,Tianjin 300457,China)

机构地区：[1]天津师范大学化学学院,天津300387 [2]天津科技大学生物工程学院,天津300457

出　　处：《广州化工》2021年第23期35-37,共3页GuangZhou Chemical Industry

基　　金：天津市大学生创新创业训练计划项目(No:201910065011;No:202010057090)。

摘　　要：硝基还原酶(NTR)是一类依赖黄素单核苷酸或黄素腺嘌呤二核苷酸的细胞质酶,可还原芳香族硝基化合物,实现相关药物的激活、解毒和相关环境污染物的生物降解;生命体中NTR含量与细胞缺氧程度直接相关。因此,NTR检测受到广泛关注。为保证NTR较高催化活性,基于工程菌制备NTR,并结合氟硼吡咯研究工作,确定了NTR含量测定方法。Nitroreductase is a kind of cytoplasmic enzyme which depends on flavin mononucleotide or flavin adenine dinucleotide.It can reduce aromatic nitro compounds to their corresponding amino compounds;realize the activation,detoxification of relative drugs and biodegradation of the relevant environmental pollutants.The content of nitroreductase in life is directly related to the degree of cell hypoxia.Therefore,the detection of nitroreductase is paid much more attention.To obtain high catalytic activity of NTR,NTR based on engineering bacteria was prepared.Combined with the research work of BODIPY,the detection method of NTR was determined.

关 键 词：硝基还原酶 氟硼吡咯 检测 

分 类 号：O657.3[理学—分析化学]