苗药透骨香的抗氧化活性研究及总黄酮、总酚酸的含量测定  

Study on Antioxidant Activity of Miao Nationality Herbal Medicine Gaultheria Yunnanensis and Determination of Total Flavones and Phenolic Acid

作　　者：高秀印 李金金 肖婷 郭正红 谢树才 刘红云 GAO Xiu-yin;LI Jin-jin;XIAO Ting;GUO Zheng-hong;XIE Shu-cai;LIU Hong-yun(Guizhou University of Traditional Chinese Medicine,Guizhou Guiyang 550025;Guizhou Medical University,Guizhou Guiyang 550025,China)

机构地区：[1]贵州中医药大学,贵州贵阳550025 [2]贵州医科大学,贵州贵阳550025

出　　处：《广州化工》2021年第23期93-96,共4页GuangZhou Chemical Industry

基　　金：黔高教发[2017]158号一流大学一流专业中药学大学生创新项目(基于5-Lox靶点的透骨香抗炎活性部位筛选);贵州省中医药管理局中医药、民族药科学技术研究(编号:QZYY2017-091);贵州省科技计划项目(基于UPLC-TOF-MS技术对民族药血盆草中止血活性部位血中移行成分的研究,编号:黔科合基础-ZK[2021]一般540)。

摘　　要：采用DPPH、ABTS自由基清除法和FRAP测定苗药透骨香各萃取层的自由基清除能力和总抗氧化能力,采用福林酚法和Al(NO_(3))_(3)-NaNO_(2)-NaOH显色法分别测定其中多酚和总黄酮含量。结果表明,乙酸乙酯萃取层的自由基清除活性和总抗氧化能力最强,其次为二氯甲烷层和正丁醇层,石油醚层和水层的活性较弱。其自由基清除能力和抗氧化活性与各萃取层中的总黄酮和总酚酸含量有关,其中乙酸乙酯萃取层的总黄酮和总酚酸含量最高,其次为正丁醇和二氯甲烷萃取层。本实验为苗药透骨香进一步深度开发、综合利用提供了理论依据。DPPH,ABTS free radical scavenging methods and FRAP were used to determine the free radical scavenging ability and total antioxidant capacity of each extraction fractions of Miao nationality herbal medicines Gaultheria yunnanensis.Folin-Ciocalteau method and Al(NO_(3))_(3)-NaNO_(2)-NaOH colorimetric method were used to determine the contents of polyphenols and total flavonoids.The results showed that the ethyl acetate extraction fraction had the strongest free radical scavenging activity and total antioxidant capacity,followed by dichloromethane fraction and n-butanol fraction,and the petroleum ether and water fractions had the weaker activity.The free radical scavenging ability and antioxidant activity were related to the contents of total flavonoids and total phenolic acids in each extraction,in which the contents of total flavonoids and total phenolic acids in ethyl acetate extraction were the highest,followed by n-butanol and dichloromethane extractions.This experiment provided a theoretical basis for the further development,comprehensive utilization and optimization of the herbal medicine.

关 键 词：抗氧化 总黄酮 总酚酸 

分 类 号：R284.1[医药卫生—中药学]