盐胁迫下枯草芽孢杆菌NCD-2对番茄促生作用及对土壤微生物群落结构的影响  

Effect of Bacillus subtilis NCD-2 on the Growth of Tomato and the Microbial Community Structure of Rhizosphere Soil Under Salt Stress

作　　者：邵美琪 赵卫松[2] 苏振贺 董丽红[2] 郭庆港[2] 马平[2] SHAO MeiQi;ZHAO WeiSong;SU ZhenHe;DONG LiHong;GUO QingGang;MA Ping(College of Plant Protection,Hebei Agricultural University,Baoding 071001,Hebei;Plant Protection Institute of Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province/Key Laboratory of IPM on Crops in Northern Region of North China,Ministry of Agriculture and Rural Affairs,Baoding 071000,Hebei)

机构地区：[1]河北农业大学植物保护学院,河北保定071001 [2]河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心/农业农村部华北北部作物有害生物综合治理重点实验室,河北保定071000

出　　处：《中国农业科学》2021年第21期4573-4584,共12页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31572051);河北省重点研发计划(19226510D);河北省农林科学院现代农业科技创新工程(2019-1-2-3);河北省自然科学基金(C2019301101)。

摘　　要：【目的】探讨盐胁迫下生防枯草芽孢杆菌(Bacillus subtilis)NCD-2菌株对番茄的促生效果及对土壤微生物群落多样性的影响,为拓宽NCD-2菌株的应用提供理论依据。【方法】采用温室盆栽试验,测定盐胁迫下NCD-2菌株对番茄株高、地上部、根部干重和鲜重的影响,并测定抗逆相关酶超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性和脱落酸(ABA)含量;采用高通量测序(Illumina MiSeq)技术测定NCD-2菌株菌悬液处理(NCD0)、100mmol·L^(-1)NaCl处理(CK100)、NCD-2菌株菌悬液+100 mmol·L^(-1)NaCl处理(NCD100)和清水处理作为对照(CK0)条件下土壤真菌、细菌群落结构,分析盐胁迫下NCD-2菌株对番茄根际土壤微生物群落结构的影响。【结果】正常条件下,经NCD-2菌株处理,番茄株高、地上部鲜重、地上部干重、根部鲜重和根部干重分别较对照增加了9.08%、10.37%、16.64%、15.42%和16.78%;在100 mmol·L^(-1)NaCl盐胁迫下,经NCD-2菌株处理,番茄株高、地上部鲜重、地上部干重、根部鲜重和根部干重分别较对照增加了16.86%、18.96%、21.32%、10.50%和23.99%。盐胁迫下,经NCD-2菌株处理,番茄体内SOD、POD、CAT活性和ABA含量分别较对照提高了50.45%、56.18%、29.55%和34.60%。细菌群落组成分析表明,在没有盐胁迫的条件下,NCD-2菌株处理后番茄根际放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)和绿弯菌门(Chloroflexi)菌群的相对丰度分别较清水对照(CK0)提高了7.28%、15.14%和23.03%;节杆菌属(Arthrobacter)、鞘氨醇单胞菌属(Sphingomonas)、微枝形杆菌属(Microvirga)和链霉菌属(Streptomyces)菌群的相对丰度分别较CK0提高了50.88%、15.31%、11.32%和16.41%。在100 mmol·L^(-1)NaCl盐胁迫下,经NCD-2菌株处理,番茄根际变形菌门(Proteobacteria)、放线菌门、厚壁菌门(Firmicutes)和芽单胞菌门(Gemmatimonadetes)菌群的相对丰度分别较单独NaCl处理(CK100)提高了6.08%、8.19%、14.11%【Objective】The objective of this study is to evaluate the growth promotion effect of Bacillus subtilis strain NCD-2 on tomato seedlings under salt stress,as well as the effect of strain NCD-2 on soil microbial community diversity.The results will be useful for expanding the application of strain NCD-2 in agricultural system.【Method】The pot experiments were conducted to evaluate the effects of strain NCD-2 treatment on stem length,aboveground fresh weight,aboveground dry weight,root fresh weight and root dry weight.The activity of resistance-related enzymes such as peroxidase(POD),superoxide dismutase(SOD),catalase(CAT)and the content of abscisic acid(ABA)were measured.High-throughput sequencing(Illumina MiSeq)technique was used to determine the bacterial and fungal community structures in the rhizosphere soil.The four treatments were set as NCD-2 strain suspension treatment(NCD0),100 mmol·L^(-1)NaCl treatment(CK100),NCD-2 strain suspension+100 mmol·L^(-1)NaCl treatment(NCD100)and water treatment as control(CK0).【Result】Under normal conditions,strain NCD-2 treatment significantly increased the biomass of tomato.The plant height,aboveground fresh weight,aboveground dry weight,root fresh weight and root dry weight were increased by 9.08%,10.37%,16.64%,15.42%and 16.78%,respectively,when compared with the control.Under salt stress,compared to the control,the plant height,aboveground fresh weight,aboveground dry weight,root fresh weight and root dry weight were increased by 16.86%,18.96%,21.32%,10.50%and 23.99%after treated with strain NCD-2,respectively.The activity of resistance-related enzymes SOD,POD,CAT and the content of ABA were increased by 50.45%,56.18%,29.55%and 34.60%after treated with strain NCD-2,respectively,when compared with the control.For bacteria community composition analysis,compared to CK0,the relative abundance of bacteria phylum Actinobacteria,Acidobacteria and Chloroflexi was increased by 7.28%,15.14%and 23.03%after treated with strain NCD-2 without salt stress,respectively.The re

关 键 词：枯草芽孢杆菌 盐胁迫 抗氧化酶活性 高通量测序 土壤微生物群落结构 

分 类 号：S154.3[农业科学—土壤学] S641.2[农业科学—农业基础科学]