大豆卵磷脂联合大豆异黄酮改善大鼠学习记忆损伤及脑血管保护作用  

Joint effects of soy lecithin and soy isoflavone on improving learning and memory impairment and cerebrovascular function in rats

作　　者：余辉艳 池亚菲 李红睿 王轩 周雪阳 肖荣 席元第 Yu Huiyan;Chi Yafei;Li Hongrui;Wang Xuan;Zhou Xueyang;Xiao Rong;Xi Yuandi(Department of Nutrition and Food Hygiene,School of Public Health,Capital Medical University,Beijing 100069,China;Department of Laboratory Animal,Capital Medical University,Beijing 100069,China;Maternal and Child Health Hospital,Beijing Shunyi District,Beijing 101300,China)

机构地区：[1]首都医科大学公共卫生学院营养与食品卫生学学系,北京100069 [2]首都医科大学实验动物部,北京100069 [3]北京市顺义区妇幼保健院医务科,北京101300

出　　处：《首都医科大学学报》2021年第6期993-999,共7页Journal of Capital Medical University

基　　金：国家自然科学基金(81973018,82003459);北京市教委科技计划一般项目(KM201910025024);达能营养宣教基金(DIC2019-02)。

摘　　要：目的观察大豆卵磷脂(soy lecithin,SL)和大豆异黄酮(soy isoflavone,SIF)改善大鼠学习记忆损伤及脑血管保护的联合作用,探讨二者的最佳联合剂量,为明确大豆活性物质的神经保护作用及其联合效应提供科学依据。方法本研究使用SPF级的成年雄性Wistar大鼠,采用两因素三水平正交实验设计分为对照组、β淀粉样肽(β-amyloid,Aβ)模型组和9个不同的SL+SIF剂量组合干预组。应用水迷宫、脑血管HE染色、酶联免疫吸附测定(enzyme-linked immunosorbent assay,ELISA)以及酶法试剂盒等方法,对大鼠学习记忆能力、脑血管病理损伤、体内氧化损伤水平以及氧化还原平衡体系等相关指标进行检测。结果Aβ模型组大鼠逃避潜伏期显著延长(P<0.05),脑血管组织形态异常改变明显,并且伴有体内丙二醛(malondialdehyde,MDA)和3-硝基酪氨酸(3-nitrotyrosine,3-NT)显著上升(P<0.05),谷胱甘肽与氧化型谷胱甘肽的比值(ratio of glutathione/oxidized glutathione,GSH/GSSG)显著下降(P<0.05)。相反,SL+SIF各干预组与Aβ模型组比较,二者对上述改变均有不同程度的保护作用。SL(40 mg·kg^(-1)·d^(-1))+SIF(50 mg·kg^(-1)·d^(-1))是改善大鼠学习记忆、保护脑血管以及调节体内氧化还原平衡体系的最佳联合剂量。结论SL+SIF可以显著改善Aβ介导的大鼠学习记忆损伤,其神经保护作用可能与二者发挥抗氧化活性拮抗Aβ介导的脑血管损伤有关。二者的神经保护作用具有协同效应,SL(40 mg·kg^(-1)·d^(-1))+SIF(50 mg·kg^(-1)·d^(-1))是大鼠神经保护的最佳联合剂量。Objective To explore the effects of soy lecithin(SL)combined with soy isoflavone(SIF)on cognitive function and cerebrovascular damage in rats with learning and memory impairment,and to explore the best combined dosage in order to provide scientific evidence of the neuroprotective effects of soybean s active substances and their combined supplementary effects.Methods:In this study,SPF-grade adult male Wistar rats were used.Using the L9(34)orthogonal experimental design,control group,β-amyloid(Aβ)group and nine different dose combinations of SIF+SL were obtained.Methods Such as water maze,cerebrovascular HE staining,enzyme-linked immunosorbent assay(ELISA),and enzymatic kits were used.The rat s learning and memory ability,cerebrovascular pathological damage,the level of oxidative damage,and the redox balance system were tested.Results Compared with the control group,the escape latency of the Aβmodel group rats was significantly prolonged(P<0.05),and the morphological changes of cerebrovascular tissue were obvious,and there were increased significant changes in malondialdehyde(MDA)and 3-nitrotyrosine(3-NT)in rats(P<0.05),the ratio of glutathione/oxidized glutathione(GSH/GSSG)decreased significantly(P<0.05).On the contrary,compared with the model group in the SL+SIF intervention group,SL+SIF played a protective role for the model group.Based on the above results,the study found that SL(40 mg·kg^(-1)·d^(-1))+SIF(50 mg·kg^(-1)·d^(-1))is the best combined dose to improve the rat s learning and memory impairment,protect the cerebral blood vessels and regulate the redox balance system.Conclusion SL+SIF can significantly improve Aβ-mediated learning and memory impairment in rats,and its neuroprotective effect may be related to the antioxidant activity of the two to antagonize Aβ-mediated cerebrovascular injury,and their neuroprotective effects of SL(40 mg·kg^(-1)·d^(-1))+SIF(50 mg·kg^(-1)·d^(-1))is the best combined dose for rats.

关 键 词：大豆卵磷脂 大豆异黄酮 联合 学习记忆损伤 Β淀粉样肽 

分 类 号：R459.3[医药卫生—治疗学]