广谱抗稻瘟病基因BSR-d1在75份北方粳稻品种中的多态性分布与表达分析  

Polymorphism Distribution and Expression Analysis of Broad-spectrum Blast Resistance Gene BSR-d1 in 75 Northern Japonica Rice Varieties

作　　者：陈晓军[1] 惠建[1] 马洪文[2] 白海波[1] 马静[2] 马斯霜 李树华[1] CHEN Xiaojun;HUI Jian;MA Hongwen;BAI Haibo;MA Jing;MA Sishuang;LI Shuhua(Agricultural Biotechnology Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002;Institute of Crop Sciences,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002)

机构地区：[1]宁夏农林科学院农业生物技术研究中心,银川750002 [2]宁夏农林科学院农作物研究所,银川750002

出　　处：《种子》2021年第11期113-116,131,共5页Seed

基　　金：宁夏农业育种专项(2018 NYYZ-0302);国家自然科学基金(31860374);宁夏农林科学院科技创新引导项目(NKY-18-06)。

摘　　要：稻瘟病是危害水稻最严重的病害之一,利用分子标记选育抗病品种是防治该病害最有效、最经济的方法。BSR-d1基因是对稻瘟病菌具有广谱抗性的一个重要基因。本研究利用PCR直接扩增的方法,研究广谱抗稻瘟病基因BSR-d1启动子的序列结构变异,同时验证其变异对靶基因的调控。结果表明,在75份北方粳稻品种中,仅有3份粳稻品种有BSR-d1基因的有利等位基因分布,此基因的突变降低过氧化物酶前体Os05g04470,Os10g39170基因的表达。Rice blast is one of the most serious diseases in rice, and it is the most effective and economical method to select and breed resistant varieties by using molecular marker selection. BSR-d1 gene is an important gene with broad-spectrum resistance to rice blast. In this study, direct PCR amplification was used to study the structural variation of the promoter of the broad-spectrum blast resistance gene BSR-d1, and to verify its regulation of target genes. The results showed that only 3 of the 76 northern japonica rice varieties had favorable allele distribution of BSR-d1 gene, and the mutation of this gene reduced the expression of peroxidase precursors Os05 g04470 and Os10 g39170 genes.

关 键 词：粳稻 稻瘟病 BSR-d1 多态 定量表达 

分 类 号：S511[农业科学—作物学]