应用ISSR分子标记绘制黔西南州梨品种DNA的指纹图谱  

DNA Fingerprinting of Pear Varieties in Qianxinan Prefecture Based on ISSR Molecular Markers

作　　者：桂腾琴 李春艳 秦燕 韦燕 王颖娟 GUI Tengqin;LI Chunyan;QIN Yan;WEI Yan;WANG Yingjuan(School of Biology and Chermistry,Xingyi Normal University for Nationalities,Xingyi Guizhou 562400,China;Research and development of biological resources,key Laboratory of national medicine,Xingyi Guizhou 562400,China)

机构地区：[1]兴义民族师范学院生物与化学学院,贵州兴义562400 [2]贵州省民族药用生物资源研究与开发重点实验室,贵州兴义562400

出　　处：《种子》2021年第11期126-131,共6页Seed

基　　金：贵州省科技厅科学技术基金项目(黔科合J字[2013]2276号);黔西南州科技计划项目(2013-1);贵州省普通高等学校青年科技人才成长项目(黔教合KY字[2019]221号);贵州省教育厅特色领域项目(黔教合KY字[2020]072)。

摘　　要：以黔西南州24个梨品种为材料,从ISSR随机引物中挑选出12条重复性好且条带清晰的引物,再从12条ISSR引物中遴选出3条核心引物UBC 825(AC)_(8)T、UBC 834(AG)_(8)YT、UBC 816(GA)_(8)T,绘制黔西南州24个梨品种的DNA指纹图谱,为梨分子身份证的构建奠定了基础。In this study, twelve primers with good repeatability and clear bands were selected from ISSR random primers to amplify 24 pear varieties in Qianxinan Prefecture, and three core primers UBC 825(AC)_(8)T, UBC 834(AG)_(8)YT and UBC 816(GA)_(8)T were further selected from 12 ISSR primers to draw the DNA fingerprints of 24 pear varieties, which laid a foundation for the construction of pear molecular identity card.

关 键 词：梨 DNA ISSR 指纹图谱 

分 类 号：Q081[生物学] S661.2[农业科学—果树学]