氟苯尼考粉中非法添加乙酰甲喹的检测方法研究  

Determination of methaquine illegaly added into florfenicol powder

作　　者：文英会[1] 邱天宝 刘占通 吴慧豪 WEN Yinghui;QIU Tianbao;LIU Zhantong;WU Huihao(College of Food and Bioengineering,Henan University of Animal Husbandry and Economy,Zhengzhou Henan 450046;Henan Animal Health Supervision Institution,Zhengzhou Henan 450008)

机构地区：[1]河南牧业经济学院食品与生物工程学院,河南郑州450046 [2]河南省兽药饲料监察所,河南郑州450008

出　　处：《现代牧业》2021年第4期1-5,10,共6页Modern Animal Husbandry

摘　　要：建立了利用高效液相色谱检测氟苯尼考粉中添加乙酰甲喹的方法。采用Waters XBridge C18色谱柱,以乙腈-水(40∶60)为流动相,流速1.0 mL/min,PDA检测器,扫描范围200~400 nm,检测波长256 nm,柱温30℃,进样量10μL。结果表明,乙酰甲喹浓度在0.002~0.04 mg/L范围内与峰面积呈良好的线性关系(r=0.9999);平均回收率为99.0%(n=6),RSD值为0.4%。本方法高效、简便、准确、可行,适用于氟苯尼考粉中非法添加乙酰甲喹的检测。A method was established to detect the methaquine in florfenicol powder with illegal addition by high performance liquid chromatography.Method Waters XBridge C18 chromatographic column was used,acetonitrile-water(40:60)was used as the mobile phase,the flow rate was controlled at 1.0 mL/min,and a PDA detector was used.The scanning wavelength was 200~400 nm and the detection chromatogram wavelength was 256 nm.The column temperature is 30℃,and the injection volume is 10μL.The results showed that the injection concentration of methaquine was in a good linear relationship with the peak area in the range of 0.002~0.04 mg/L(R 2=0.9999);the average recovery rate was 99.1%(n=6),and the RSD value was 0.46%.This method is efficient,simple,accurate,and feasible,and is suitable for the detection of the illegal addition of methaquine in florfenicol powder.

关 键 词：高效液相色谱法 氟苯尼考粉 乙酰甲喹 

分 类 号：TS251[轻工技术与工程—农产品加工及贮藏工程]