Strain Pseudomonas putida PAO-1 Isolate with Polyphosphate Accumulating and Elongation Ability  

作　　者：DONG Xuetong TIAN Qing ZHU Yanbin LI Fang YANG Bo LIU Yanbiao 董雪彤;田晴;朱艳彬;李方;杨波;刘艳彪(College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China)

机构地区：[1]College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China

出　　处：《Journal of Donghua University(English Edition)》2021年第6期539-548,共10页东华大学学报（英文版）

基　　金：National Natural Science Foundation of China(No.21777024);National Key Research and Development Project,China(No.2019YFC0408503)。

摘　　要：Filamentous bacteria(FB)overgrowth is an important cause of sludge bulking in wastewater treatment plants(WWTPs).However,to date,methods for the cultivation and preservation of isolated FB in the laboratory have not been completely described.Furthermore,research on whether FB can function as phosphorus accumulating organisms(PAOs)is limited.In this study,a pure strain,a Pseudomonas putida PAO-1(P.putida PAO-1)isolate with phosphorus removal functions was isolated from the biofilm of an alternating anaerobic/aerobic biofilter(AABF),and its physiological characteristics were studied.Nitrate or nitrite could be used by the strain P.putida PAO-1 as electron acceptors for denitrification during phosphorus anoxic uptake,and 0.63 mg NO-3-N was consumed to reduce 1 mg soluble orthophosphate(SOP)by P.putida PAO-1.The strain P.putida PAO-1 consumed phosphorus within the optimal pH range of 6 to 8 and the temperature range of 25℃to 35℃.Cell deformity was a main morphological trait of the strain P.putida PAO-1,and it could elongate(with an elongation rate of 300%-500%)when it was subjected to oligotrophic or high-salt stress(15 g·L-1 NaCl).The findings in this study provide a microbiological reference for understanding the special characteristics of a denitrifying PAO.

关 键 词：Pseudomonas putida PAO-1 isolate(P.putida PAO-1) ELONGATION ACCLIMATION denitrification phosphorus accumulating organism(PAO) 

分 类 号：X703.1[环境科学与工程—环境工程]