核酸检测技术进展及其在SARS-CoV-2核酸检测中的应用  

Progress in Nucleic Acid Detection Technology and Its Application in SARS-CoV-2 Nucleic Acid Detection

作　　者：孟德豪 张艳芬[2] 孙玉淼 高旭 马宁[1,3,4,6] MENG De-Hao;ZHANG Yan-Fen;SUN Yu-Miao;GAO Xu;MA Ning(Department of Biochemistry and Molecular Biology,School of Basic Medical Sciences,Harbin Medical University,Harbin 150081,China;Department of Clinical Laboratory,The Second Affiliated Hospital,Harbin Medical University,Harbin 150000,China;Heilongjiang Institute of Basic Medicine,Harbin 150086,China;Translational Medicine Research and Cooperation Center of Northern China,Harbin Medical University,Harbin 150081,China;Key Laboratory of Preservation of Human Genetic Resources and Disease Control in China,Ministry of Education,Harbin 150081,China;Key Laboratory of Genetically Modified Model Animals of Heilongjiang Province,Harbin 150081,China)

机构地区：[1]哈尔滨医科大学基础医学院生物化学与分子生物学教研室,哈尔滨150081 [2]哈尔滨医科大学附属第二医院检验科,哈尔滨150000 [3]黑龙江省基础医学研究所,哈尔滨150086 [4]哈尔滨医科大学北方转化医学研究合作中心,哈尔滨150081 [5]教育部中国遗传资源保护与疾病防控重点实验室,哈尔滨150081 [6]黑龙江省遗传改变模式动物重点实验室,哈尔滨150081

出　　处：《生物化学与生物物理进展》2021年第11期1290-1300,共11页Progress In Biochemistry and Biophysics

基　　金：黑龙江省自然科学基金(H2018006);黑龙江省教育科学规划重点课题(GJB1320181);黑龙江省高等教育教学改革(重点)项目(SJGZ20200109);哈尔滨医科大学附属第二医院中青年创新科学研究基金(2020);哈尔滨医科大学少帅揭榜项目(HMUMIF21025)资助.

摘　　要：2020年初至今,新型冠状病毒肺炎(COVID-19)疫情仍在全球多个国家流行,给全球公共卫生安全造成了严重威胁.中国在应对COVID-19的实践中,最先完成病毒核酸测序并共享序列信息,最早有效控制疫情蔓延、恢复生产,并在病毒作用人体机制研究、疫苗研发、中和性抗体发现等环节均处于世界前沿.其中,针对病毒核酸的检测工作——试剂盒研发、检测配套设备研制、10合1混检策略及相关政策制订,有效提高了疫情防控效率、保障了百姓健康.本文就严重急性呼吸系统综合症冠状病毒2(SARS-CoV-2)核酸检测多种方法的原理、优劣势及其配套设备等进行综述.Up to now,the COVID-19 is still prevalent in many countries around the world,and threatens health security of global public seriously.China not only developed the reagent kits for detecting of SARS-CoV-2 nucleic acids and testing equipment,but also established 10 in 1 mixed detection strategy and related policies which have improved the efficiency of epidemic prevention and control,and ensured the health of the people.This review summarizes the principles,advantages,disadvantages and supporting equipment of various methods for the detection of SARS-CoV-2.

关 键 词：新型冠状病毒 核酸检测 CRISPR 

分 类 号：Q5[生物学—生物化学] Q7