固相萃取/液相色谱-电喷雾串联质谱法测定水中噻菌灵  

Determination of Thiabendazole in Water by Solid Phase Extraction/Liquid Chromatography-Electrospray Ionization Tandem Mass Spectrometry

作　　者：陈峰[1] 张宝锋 何平[1] 王奕奕[1] 周姗[1] 董翠维 CHEN Feng;ZHANG Bao-feng;HE Ping;WANG Yi-yi;ZHOU Shan;DONG Cui-wei(Hangzhou Environmental Monitoring Center Station,Hangzhou 310007,China)

机构地区：[1]杭州市环境监测中心站,浙江杭州310007

出　　处：《中国给水排水》2021年第22期109-112,共4页China Water & Wastewater

基　　金：杭州市农业和社会发展科研项目(20191203B66);杭州市农业和社会发展科研项目(20191203B65);杭州市“131”中青年人才培养计划(2016-2020年)。

摘　　要：研究了固相萃取/液相色谱-电喷雾串联质谱联用技术(SPE/LC-ESI-MS/MS)分析水中噻菌灵的方法。样品经SPE小柱萃取后,采用Agilent ZORBAX SB-C18色谱柱,以0.2%甲酸乙腈和0.2%甲酸水为流动相进行梯度洗脱,用电喷雾离子源电离,正离子多反应监测模式下进行定性、定量分析。噻菌灵在0~100μg/L浓度范围内线性良好,相关系数达0.999 2,方法检出限为0.16 ng/L;高、中、低三个加标水平下(5.0、20.0和100 ng)实际样品加标回收率范围为74.7%~86.5%,相对标准偏差范围为6.1%~12.9%。应用本方法检测了杭州市68个地表水样品的噻菌灵浓度,其中25个样品有检出。A method of solid phase extraction/liquid chromatography-electrospray ionization tandem mass spectrometry(SPE/LC-ESI-MS/MS)for determination of thiabendazole in surface water was explored.The samples were extracted by SPE column.Liquid chromatography separation was performed on an Agilent ZORBAX SB-C18 column with a gradient elution of 0.2%formic acid acetonitrile and 0.2%formic acid as the mobile phases.Electrospray ionization and qualitative and quantitative analysis were carried out under the positive ion multi-reaction monitoring mode.Good linearity was observed in the range of 0-100μg/L thiabendazole with correlation coefficient 0.9992,and the limit of detection(LOD)was 0.16 ng/L.At three different standard addition levels(5.0,20.0 and 100 ng),average recovery rates of thiabendazole in practical samples ranged from 74.7%to 86.5%with RSD between 6.1%and 12.9%.The method was applied to detect thiabendazole concentration in 68 samples of surface water in Hangzhou,and thiabendazole in 25 samples was detected.

关 键 词：地表水 固相萃取 液相色谱-电喷雾串联质谱 噻菌灵 

分 类 号：TU991[建筑科学—市政工程]