高温高湿环境下重度中暑模型猪的建立  

Establishment of a severe heatstroke pig model under high-temperature and high-humidity environment

作　　者：杨彦文 张静[1] 毛玉玲 倪军[1] 毛利宾 姚璐[1] 王会英[1] 刘秋明 马珍珍[1] Yang Yanwen;Zhang Jing;Mao Yuling;Ni Jun;Mao Libin;Yao Lu;Wang Huiying;Liu Qiuming;Ma Zhenzhen(The 83^(rd)Army Hospital of Chinese PLA,Xinxiang 453000,Henan Province,China)

机构地区：[1]中国人民解放军陆军第八十三集团军医院,河南省新乡市453000

出　　处：《中国组织工程研究》2022年第17期2678-2684,共7页Chinese Journal of Tissue Engineering Research

基　　金：军队后勤科研计划项目(CLJ20J026),项目负责人:倪军。

摘　　要：背景:重度中暑小型动物模型由于体积小,限制了血液净化等临床干预手段实施,而大型动物模型国内外多采用狒狒等灵长类动物,成本高。研究采用的巴马小型猪,其遗传稳定性较好,与人的体温调节、心血管反应相近,容易获取,成本低廉。目的:建立高温高湿环境中猪重度中暑模型,为实施血液净化等干预治疗提供实验平台。方法:雄性实验小型猪12头,随机分2组,分组实验条件为:A组温度35-37℃(做对照);B组温度44-47℃(建立高温高湿环境中猪重度中暑模型),两组湿度均为(65±5)%;实验观察时间为4 h。详细记录小型猪的自主状态、结膜及提肛反射变化;采用多通道生理记录仪连续监测生命体征;造模前后检测实验动物全血细胞计数、肝肾功能、肌酸激酶等变化;实验终止后,取实验动物的重要脏器组织行病理检查。实验方案经解放军第八十三集团军医院医学伦理委员会批准。结果与结论:(1)热暴露前(T-1 h)、热暴露(造模)结束实验前后B组猪自主行为和生命体征出现明显变化,B组小型猪实验后全血白细胞、活化部分凝血活酶时间、凝血酶原时间、血钾、肌酐及尿素氮、转氨酶及肌酸激酶水平均出现明显异常(均P<0.05或P<0.01),6头小型猪尿常规均出现不同程度的变化,病理形态学观察显示多个脏器损伤;A组猪自主行为无显著异常,生命体征及实验室检查结果有轻微改变;(2)结果表明,实验动物猪在温度44-47℃、湿度(65±5)%的恒温恒湿环境中4 h,自主行为、生命体征及实验室检查均出现显著异常,达到重度中暑标准;实验动物猪重度中暑模型可重复性好,能满足临床重度中暑血液净化等研究需求。BACKGROUND:Due to the small size of severe heatstroke small animal models,there is a limitation to the implementation of blood purification and other clinical interventions,while mostly used large animal models,such as baboon,are expensive.The Bama miniature pig used in this study has good genetic stability,and is similar to the human body in terms of body temperature regulation and cardiovascular response,which is also easy to obtain and has low cost.OBJECTIVE:To prepare the pig model of severe heatstroke under high temperature and high humidity environment,thereby providing an experimental platform for blood purification and other intervention treatments.METHODS:Twelve male experimental miniature pigs were randomly divided into two groups(n=6 per group):group A(control)and group B in which a pig model of severe heatstroke was established.The experimental conditions are as follows:group A:temperature 35-37°C;group B:temperature 44-47°C;the humidity was(65±5)%in both groups;and the experiment in each group lasted for 4 hours.The autonomic state,conjunctiva and levator ani reflex were recorded in detail.The vital signs of experimental animals were continuously monitored by multi-channel physiological recorder.The whole blood cell count,liver and kidney function,creatine kinase,blood coagulation function and urine routine were detected before and after modeling.After the end of the experiment,the important organs and tissues of the experimental animals were taken for pathological examination.The study protocol was approved by the Medical Ethics Committee of the 83 rd Army Hospital of Chinese PLA.RESULTS AND CONCLUSION:Pigs in the two groups were exposed to different environmental conditions for 4 hours.There were significant changes in autonomous behaviors and vital signs of pigs in group B before and after the experiment,and also in laboratory examination results,including whole blood white blood cells,activated partial thromboplastin time,prothrombin time,blood potassium,creatinine and urea nitrogen,aminotrans

关 键 词：重度中暑 大型动物 多脏器衰竭 模型 猪 高温 高湿 热损伤 

分 类 号：R446[医药卫生—诊断学] R496[医药卫生—临床医学]