有氧运动干预非酒精性脂肪肝小鼠肝脏JAK2/STAT5信号通路的变化  

Effects of aerobic exercises on JAK2/STAT5 signal pathway in the liver of mice with non-alcoholic fatty liver disease

作　　者：张树玲[1] 李军汉[1] 王佳倩 李亚龙 王纯[1] Zhang Shuling;Li Junhan;Wang Jiaqian;Li Yalong;Wang Chun(Chengdu Sport University,Chengdu 610041,Sichuan Province,China)

机构地区：[1]成都体育学院,四川省成都市610041

出　　处：《中国组织工程研究》2022年第17期2690-2695,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金项目(31900846),项目负责人:李军汉;四川省科技厅重点研发项目(2017SZ0018),项目负责人:王纯。

摘　　要：背景:酪氨酸激酶2/信号传导及转录激活因子5信号通路在脂质代谢中起重要作用,运动有效预防与治疗非酒精性脂肪肝与改善内脏脂质沉积和脂质代谢密切相关。目的:探讨有氧运动对非酒精性脂肪肝小鼠肝脏酪氨酸激酶2/信号传导及转录激活因子5信号通路的影响及可能作用机制。方法:6周龄C57BL/6雄性小鼠48只,分为普通膳食组及高脂膳食诱导组(n=24),第10周末2组各随机抽取4只进行油红O染色观察肝脏病理形态变化。确定造模成功后,普通膳食组随机分为普通膳食+安静组、普通膳食+运动组(n=10);高脂膳食诱导组随机分为非酒精性脂肪肝+安静组、非酒精性脂肪肝+运动组(n=10),连续干预8周,直至实验结束。观察小鼠肝脏病理形态变化,检测小鼠肝脏组织泌乳素受体、肝脏酪氨酸激酶2、信号传导及转录激活因子5a、肝脏酪氨酸激酶2磷酸化、信号传导及转录激活因子5磷酸化蛋白表达量。结果与结论:(1)与普通膳食+安静组相比,非酒精性脂肪肝+安静组肝细胞脂肪变性加重,肝脏内酪氨酸激酶2/信号传导及转录激活因子信号通路蛋白表达量降低;(2)与非酒精性脂肪肝+安静组相比,非酒精性脂肪肝+运动组的肝细胞脂肪变性减轻,肝脏内酪氨酸激酶2/信号传导及转录激活因子5信号通路蛋白表达量升高;(3)肝脏病理形态指标与催乳素受体、肝脏酪氨酸激酶2、肝脏酪氨酸激酶2磷酸化、信号传导及转录激活因子5磷酸化均呈显著负相关(P<0.05);(4)提示有氧运动干预可通过调节非酒精性脂肪肝小鼠肝脏酪氨酸激酶2/信号传导及转录激活因子5信号通路,改善肝内脂质沉积及肝脏脂肪变性程度。BACKGROUND:The Janus kinase 2/signal transducer and activator of transcription 5(JAK2/STAT5)signaling pathway plays an important role in lipid metabolism.The effective prevention and treatment of non-alcoholic fatty liver by exercise is closely related to the improvement of visceral lipid deposition and lipid metabolism.OBJECTIVE:To explore the effect of aerobic exercise on the JAK2/STAT5 signaling pathway in the liver of non-alcoholic fatty liver mice and its possible mechanism.METHODS:Forty-eight C57 BL/6 male mice aged 6 weeks were randomly divided into a normal diet group(n=24)and a high-fat diet induction group(n=24).On the 10;weekend,four mice were randomly selected from each group for oil red O staining to observe the pathological morphological changes of the liver.After successful modeling,the normal diet group was randomly divided into a normal diet+quiet group(n=10)and a normal diet+exercise group(n=10);the high-fat diet induction group was randomly divided into a non-alcoholic fatty liver+quiet group(n=10)and a non-alcoholic fatty liver+exercise group(n=10),followed by 8 weeks of intervention until the end of the experiment.Liver pathological changes were observed,and prolactin receptor,JAK2,and phosphorylation of JAK2 and STAT5 in the liver of mice were detected.RESULTS AND CONCLUSION:Compared with the normal diet+quiet group,hepatic steatosis was increased and the protein expression of JAK2/STAT5 signaling pathway in the liver was decreased in the non-alcoholic fatty liver+quiet group.Compared with the non-alcoholic fatty liver+quiet group,hepatic steatosis was decreased and the protein expression of JAK2/STAT5 signaling pathway in the liver was increased in the non-alcoholic fatty liver+exercise group.The liver pathological indicators were significantly negatively correlated with prolactin receptor,JAK2,and phosphorylation of JAK2/STAT5(P<0.05).To conclude,aerobic exercise intervention can improve liver lipid deposition and the degree of hepatic steatosis by regulating the JAK2/STAT5 signaling pathw

关 键 词：有氧运动 非酒精脂肪肝 酪氨酸激酶2/信号传导及转录激活因子5信号通路(JAK2/STAT5) 磷酸化 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学]