用于疫苗生产的人二倍体细胞研究进展  

Research Progress of Human Diploid Cells for Vaccine Production

作　　者：肖云喜 张俊河[1,2,3] 杨雯雯 程洪伟 XIAO Yun-xi;ZHANG Jun-he;YANG Wen-wen;CHENG Hong-wei(Institutes of Health Central Plains,Xinxiang Medical University,Xinxiang 453003,China;Department of Biochemistry and Molecular Biology,Xinxiang Medical University,Xinxiang 453003,China;Henan International Joint Laboratory of Recombinant Pharmaceutical Protein Expression System,Xinxiang Medical University,Xinxiang 453003,China)

机构地区：[1]新乡医学院健康中原研究院,新乡453003 [2]新乡医学院生物化学与分子生物学系,新乡453003 [3]河南省重组药物蛋白表达系统国际联合实验室,新乡453003

出　　处：《中国生物工程杂志》2021年第11期74-81,共8页China Biotechnology

基　　金：国家自然科学基金(U1604193);河南省重点研发与推广专项(科技攻关)(212102310634)资助项目。

摘　　要：人二倍体细胞(human diploid cells,HDCs)作为制备疫苗的重要培养细胞基质备受人们的关注。由于人二倍体细胞与人类基因组相同、无外源因子、对多种病毒易感性、无潜在致瘤性、所制备的人二倍体疫苗(human diploid cell vaccine,HDCV)具有良好的免疫原性和安全性,适合于疫苗的工业化生产。当前,人群中使用的灭活疫苗、减毒疫苗或亚单位疫苗等均依赖于原代细胞、传代细胞和人二倍体细胞,其中用于疫苗制备的人二倍体细胞主要有WI-38、MRC-5、2BS和KMB-17等细胞系。然而,人二倍体细胞为有限性细胞,细胞的来源和培养技术等存在某些缺陷,进而影响其应用。综述了用于疫苗生产的人二倍体细胞及其疫苗制备技术的研究进展,并分析了存在的问题及改进策略。Human diploid cells(HDCs)have attracted much attention as an important culture cell matrix for vaccine preparation.Since human diploid cells are the same as the human genome without exogenous factor,susceptible to a variety of viruses,and without potential tumorigenicity,and the preparation of human diploid cell vaccine(HDCV)has good immunogenicity and safety,they are suitable for industrial production of vaccine.Currently,the inactivated,attenuated or subunit vaccines used in the population all rely on primary cells,continuous cells and human diploid cells,of which WI-38,MRC-5,2 BS and KMB-17 cell lines are the main human diploid cells used in vaccine preparation.However,human diploid cells are limited cells,and there are some defects in cell sources and culture techniques,which further affect their application.In this paper,the research progress of human diploid cells used in vaccine production and vaccine preparation technology were reviewed,and the existing problems and improvement strategies were analyzed.

关 键 词：人二倍体细胞 疫苗 细胞系 疫苗制备技术 

分 类 号：Q819[生物学—生物工程]