怀中1号鲜地黄化学成分研究  

Chemical constituents of the fresh roots of Huaizhong NO.1 Rehmannia glutinosa

作　　者：范锡玲 刘晏灵 曹彦刚 任英杰 王梦娜 陈旭 何晨 郑晓珂[1,2] 冯卫生 FAN Xi-ling;LIU Yan-ling;CAO Yan-gang;REN Ying-jie;WANG Meng-na;CHEN Xu;HE Chen;ZHENG Xiao-ke;FENG Wei-sheng(School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China;The Engineering and Technology Center for Chinese Medicine Development of Henan Province China,Zhengzhou 450046,China)

机构地区：[1]河南中医药大学药学院,河南郑州450046 [2]河南省中药开发工程技术研究中心,河南郑州450046

出　　处：《药学学报》2021年第11期3097-3103,共7页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划:中医药现代化研究重点专项-地黄特色中药材产业链关键技术研究(2017YFC1702800);河南省重大科技专项:地黄、山药、牛膝豫产道地药材品质保障关键技术与品质特色研究(171100310500)。

摘　　要：采用Toyopreal HW-40C、Sephadex LH-20、硅胶和半制备液相等多种色谱学技术从怀中1号鲜地黄中分离得到20个化合物。根据理化性质与波谱数据鉴定其结构,分别为3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one(1)、3-氨基-2-吡啶乙醇(2)、(5’S)-2-oxo-N-phenylpyrrolidine-3-carboxamide (3)、焦谷氨酸甲酯(4)、吲唑(5)、尿苷(6)、腺苷(7)、rehmanalkaloid C (8)、1-(3-乙基苯基)-1,2-乙二醇(9)、(3-乙基苯基)-1,2-乙二醇(10)、2-羟甲基苯基-1-O-β-D-葡萄糖苷(11)、玉叶金花苷酸甲酯(12)、11-methylforsythide (13)、7-去氧栀子新苷(14)、1-O-α-L-鼠李糖苷(1→6)-β-D-吡喃葡萄糖苷(15)、6-deoxy-D-mannono-1,4-lactone (16)、富马酸单甲酯(17)、daphneresinol (18)、二氢槲皮素(19)、rhamnopyranosyl vaniloyl (20)。其中化合物1为新化合物,命名为3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one,化合物2和3为新天然产物,化合物4、5、9~11、13、14以及16~19是从地黄属中首次分离得到的。Twenty compounds were isolated and purified from the fresh roots of Huaizhong NO.1 Rehmannia glutinosa by various chromatographic techniques such as Toyopreal HW-40 C, Sephadex LH-20, silica gel and semi-preparative HPLC. Their structures were identified by analysis of physicochemical properties and spectral data, and determined as 3-methyl-2-(hydroxymethyl)-4 H-pyrone-4-one(1), 3-amino-2-pyridine ethanol(2),(5’S)-2-oxo-N-phenylpyrrolidine-3-carboxamide(3), L-pyroglutamic acid methyl ester(4), indazole(5), uridine(6),adenosine(7), rehmanalkaloid C(8), 1-(3-ethylphenyl)-1,2-ethanediol(9),(3-ethylphenyl)-1,2-ethanediol(10), 2-hydroxymethylphenyl-1-O-β-D-glucopyranoside(11), mussaenoside(12), 11-methylforsythide(13), 7-deoxygardoside(14), 1-O-α-L-rhamnopyranosyl(1→6)-β-D-glucopyranoside(15), 6-deoxy-D-mannono-1, 4-lactone(16),monoethylfumarate(17), daphneresinol(18), dihydroquercetin(19), rhamnopyranosyl vaniloyl(20). Compound 1 is a new compound, named as 3-methyl-2-(hydroxymethyl)-4 H-pyrone-4-one, compounds 2 and 3 are new natural products, and compounds 4, 5, 9-11, 13, 14 and 16-19 are isolated from Rehmannia for the first time.

关 键 词：玄参科 鲜地黄 化学成分 3-methyl-2-(hydroxymethyl)-4H-pyrone-4-one 3-氨基-2-吡啶乙醇 (5’S)-2-oxo-N-phenylpyrrolidine-3-carboxamide 

分 类 号：R284[医药卫生—中药学]