基于多指标成分含量测定探索黄芩药材质量综合评价模式  

Comprehensive quality evaluation of Scutellaria baicalensis Georgi by determination of multi-component content

作　　者：徐境荣 于钦川 赖莉 王淑美 罗国安[4] 谢媛媛 XU Jing-rong;YU Qin-chuan;LAI Li;WANG Shu-mei;LUO Guo-an;XIE Yuan-yuan(College of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Research Center for Quality Engineering Technology of Traditional Chinese Medicine,Guangzhou 510006,China;Key Laboratory of Digitalized Quality Evaluation Technology of Traditional Chinese Medicine,State Administration of Traditional Chinese Medicine,Guangzhou 510006,China;Department of Chemistry,Tsinghua University,Beijing 100084,China)

机构地区：[1]广东药科大学中药学院,广东广州510006 [2]广东省中药质量工程技术研究中心,广东广州510006 [3]国家中医药管理局中药数字化质量评价技术重点研究室,广东广州510006 [4]清华大学化学系,北京100084

出　　处：《药学学报》2021年第11期3141-3152,共12页Acta Pharmaceutica Sinica

基　　金：国家重点研发计划“中医药现代化研究”重点专项课题资助(2017YFC1700800)。

摘　　要：本研究基于多指标成分含量测定探索黄芩药材质量综合评价模式,采用高效液相色谱(HPLC)建立黄芩中8种黄酮类成分同时定量分析的方法,建立以黄芩苷为内参比物质的一测多评法(QAMS);采用2,2’-联氨-双(3-乙基苯并噻唑啉-6-磺酸)二胺盐(ABTS+)法测定不同批次黄芩药材抗氧化活性,并以这8种成分的含量与抗氧化活性效价进行灰色关联度分析,评价不同成分对黄芩药材抗氧化活性的贡献;比较不同产地、不同商品规格、不同采收年限黄芩药材的综合质量。结果表明,以黄芩苷为内参比物质测得的各指标成分相对校正因子系统适应性良好,QAMS法测定结果与外标法无显著差异;黄芩苷等8种指标成分与黄芩药材抗氧化活性关联性较强,灰色关联系数在0.772 4~0.808 6之间,其中黄芩苷、汉黄芩苷和粘毛黄芩素Ⅲ等的含量与药材抗氧化活性负相关,而黄芩素、汉黄芩素、千层纸素A、去甲汉黄芩素苷和千层纸素A-7-O-β-D葡萄糖醛酸苷等的含量则与药材抗氧化活性正相关。黄酮苷与黄酮苷元含量比值(G/A)结合药材性状特征可作为划分不同品类黄芩药材的评价指标。G/A小于10为枯芩, G/A大于10为子芩或含有枯心的子芩。本研究所建立的QAMS简便、准确,可用于黄芩8种黄酮类成分含量测定,结合ABTS+抗氧化活性与灰色关联度分析法建立了基于生物活性的黄芩质量综合评价模式,为建立符合中药自身特色的质量标准提供了新的思路和方法。A model for quality evaluation was developed by determination of multi-index components in Scutellaria baicalensis Georgi. Eight flavonoids in Scutellaria baicalensis Georgi were quantified by high performance liquid chromatography(HPLC), and a quantitative analysis of multi-components with a single marker(QAMS) method with baicalin as the internal reference substance was established. The 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt(ABTS+) method was applied to determine the antioxidant activity of different batches of Scutellaria baicalensis Georgi. Grey relevance analysis was conducted to calculate the grey correlation coefficients of each antioxidant in the herb. The holistic quality of 75 batches of collected samples from different product regions was evaluated to distinguish different commercial types of Scutellaria baicalensis Georgi.The relative correction factor(RCF) of each index component with baicalin as the internal reference indicated good applicability, and there was no significant difference in assay results between the QAMS method and the external standard method(ESM). The results of grey correlation analysis showed that the content of eight index components including baicalin correlated strongly with the antioxidant activity of Scutellaria baicalensis Georgi.The calculated grey relational coefficient ranged from 0.772 4 to 0.808 6. The content of oroxylin A-7-O-β-D-glucuronide, norwogonin-7-O-β-D-glucuronide, baicalein, wogonin and oroxylin A showed positive correlations with the antioxidant potency. In contrast, baicalin, wogonoside and viscidulin Ⅲ content exhibited negative correlations.The ratio of the flavonoid glycosides and flavonoid glycoside aglycones were found to be a critical factor to distinguish the different types of commercial herbs. The crude herbs could be characterized as Scutellaria baicalensis pith-decayed with a G/A ratio less than 10, and the ratio in Scutellaria baicalensis pith-not decayed should be more than 10. The QAMS method established

关 键 词：黄芩 黄芩苷 一测多评 相对校正因子 抗氧化 灰色关联度分析 

分 类 号：R917[医药卫生—药物分析学]