磷酸化53BP1在乳腺癌中的表达及其意义  

Expression and significance of phosphorylated 53BP1 in breast cancer

作　　者：范明江[1] 闫鲁霞 徐蕾 张园[4] 熊廷川[4] 朱长军[2,3] FAN Mingjiang;YAN Luxia;XU Lei;ZHANG Yuan;XIONG Tingchuan;ZHU Changjun(First People′s Hospital of Kashgar,Kashgar 844000,Xinjiang Uygur Autonomous Region,China;College of Life Sciences,Tianjin Normal University,Tianjin 300387,China;Tianjin Key Laboratory of Animal and Plant Resistance,Tianjin Normal University,Tianjin 300387,China;Institute of Cancer Research,The 3rd Affiliated Teaching Hospital of Xinjiang Medical University(Affiliated Cancer Hospital),Urumqi 830011,China)

机构地区：[1]新疆喀什地区第一人民医院,新疆维吾尔自治区喀什844000 [2]天津师范大学生命科学学院,天津300387 [3]天津师范大学天津市动植物抗性重点实验室,天津300387 [4]新疆医科大学第三临床医院(附属肿瘤医院)肿瘤防治研究所,乌鲁木齐830011

出　　处：《天津师范大学学报（自然科学版）》2021年第6期13-17,共5页Journal of Tianjin Normal University：Natural Science Edition

基　　金：新疆维吾尔自治区科技支疆项目(2017E0263);天津市科技支撑计划重点资助项目(18YFZCSY00100).

摘　　要：为了探究P53结合蛋白1(53BP1)磷酸化在乳腺癌发生、发展中的作用,选用乳腺癌组织芯片,该芯片含有220例乳腺癌组织和8例非癌乳腺组织,通过免疫组织化学染色实验检测,应用Image-Pro Plus荧光分析软件对图像进行分析,结合临床病例资料,研究磷酸化53BP1在乳腺癌组织中的表达.结果发现,磷酸化53BP1在乳腺癌组织中的表达水平显著高于非癌乳腺组织中的表达水平,在不同TNM分期、病理分级以及不同类型的乳腺癌组织中的平均表达量均显著高于非癌乳腺组织中的表达量(P<0.001).这些结果表明,磷酸化53BP1具有潜在的乳腺癌发生与发展的分子标志物的特性.To investigate the role of P53 binding protein 1(53BP1)phosphorylation in the occurrence and development of breast cancer,the breast cancer tissue microarray containing 220 cases of breast cancer tissues and eight cases of non-cancer tissues was adopted as experimental material.Immunohistochemical staining test was performed and the images were analyzed by Image-Pro Plus fluorescence analysis software.The expression of phosphorylated 53BP1 in breast cancer tissues was studied combined with clinical case data.The results showed that the expression level of phosphorylated 53BP1 in breast cancer tissues was significantly higher than that in non-cancer tissues.The expression level of phosphorylated 53BP1 in different TNM stages,pathological grades and different types of breast cancer was all significantly higher than that in non-cancer tissues(P<0.001).The above results suggest that phosphorylation of 53BP1 has the characteristics of potential molecular marker for the occurrence and development of breast cancer.

关 键 词：53BP1 磷酸化 乳腺癌 组织芯片 免疫组织化学染色 

分 类 号：Q71[生物学—分子生物学] Q28