基于HPLC-Q-TOF-MS技术鉴定热毒宁注射液中活性成分  

Identification of Active Components in Reduning Injection Based on HPLC-Q-TOF-MS Technology

作　　者：徐玉琴 刘娟 欧阳婷 卿志星 刘红宇[1] XU Yuqin;LIU Juan;OUYANG Ting;QING Zhixing;LIU Hongyu(The First Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410007,China;Hunan Agricultural University,Changsha,Hunan 410128,China)

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南农业大学,湖南长沙410128

出　　处：《湖南中医药大学学报》2021年第12期1869-1875,共7页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金面上项目(82174069);湖南省教育厅科学研究重点项目(18A216);湖南中医药大学药学一流学科开放基金重点项目(2021YX02)。

摘　　要：目的利用高效液相色谱-四级杆飞行时间串联质谱(HPLC-Q-TOF-MS)技术快速鉴定热毒宁注射液中主要活性成分,阐明热毒宁注射液的物质基础及微量成分。方法采用HPLC-Q-TOF-MS技术首先研究绿原酸类、多酚类化合物的质谱裂解规律,同时结合文献,利用已知的质谱裂解规律推测热毒宁注射液中主要活性成分的结构。结果共鉴定热毒宁注射液中40个化合物,包括14个绿原酸类化合物、13个环烯醚萜类化合物、13个有机酸类化合物,其中24个化合物首次在热毒宁注射液中报道。结论热毒宁注射液中主要活性成分为绿原酸、环烯醚萜和有机酸类化合物。Objective To identify the main active ingredients quickly in Reduning injection by high performance liquid chromatography quadrupole time of flight tandem mass spectrometry(HPLC-Q-TOF-MS)technology,and to clarify the material basis and trace components of Reduning injection.Methods The HPLC-Q-TOF-MS technique was first employed to investigation the fragmentation pathways of chlorogenic acids and polyphenols.And then,the structures of the main active ingredients in Reduning injection were speculated using the well-investigated fragmentation pathways of standards combined with the literatures.Results A total of 40 compounds,which including 14 chlorogenic acid,13 iridoid,and 13 organic acid type compounds were identified from Reduning injection.Among them,24 compounds were reported for the first time in Reduning injection.Conclusion The chlorogenic acids,iridoids and organic acid compounds were the main active ingredients of Reduning injection.

关 键 词：热毒宁注射液 高效液相色谱-四级杆飞行时间串联质谱 活性成分 质谱裂解规律 结构解析 

分 类 号：R284.1[医药卫生—中药学]