UPLC-MS/MS法同时测定杞菊地黄胶囊中7种有效成分  

Simultaneous Determination of Seven Effective Components in Qiju Dihuang Capsule by UPLC-MS/MS

作　　者：王珊[1] 刘红梅[1] 刘兵[1] 谭文波 童巧珍[2] 刘湘丹[2] WANG Shan;LIU Hongmei;LIU Bing;TAN Wenbo;TONG Qiaozhen;LIU Xiangdan(The Second Affiliated Hospital of Hunan University of Chinese Medicine,Changsha,Hunan 410005,China;College of Pharmacy,Hunan University of Chinese Medicine,Changsha,Hunan 410208,China)

机构地区：[1]湖南中医药大学第二附属医院,湖南长沙410005 [2]湖南中医药大学药学院,湖南长沙410208

出　　处：《湖南中医药大学学报》2021年第12期1876-1880,共5页Journal of Hunan University of Chinese Medicine

基　　金：财政部和农业农村部:国家现代农业产业技术体系资助项目(CARS-21);湖南省现代农业产业技术体系建设专项。

摘　　要：目的建立超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定杞菊地黄胶囊中莫诺苷、绿原酸、马钱苷、木犀草苷、丹皮酚、23-乙酰泽泻醇C、23-乙酰泽泻醇B的含量。方法采用ACQUITY UPLC■HSS T3(100 mm×2.1 mm,1.8μm)为色谱柱,乙腈-0.1%甲酸水溶液为流动相,梯度洗脱,流速为0.3 mL·min^(-1);采用电喷雾电离源(ESI)、多反应离子监测(MRM)模式对7种有效成分进行测定。结果每粒杞菊地黄胶囊样品中莫诺苷含量为0.28~0.35 mg,绿原酸含量为0.08~0.11 mg,马钱苷含量为0.30~0.48 mg,木犀草苷含量为0.21~0.48 mg,丹皮酚含量为0.62~0.76 mg,23-乙酰泽泻醇C含量为0.04~0.08 mg,23-乙酰泽泻醇B含量为0.15~0.32 mg。7种有效成分相关系数为0.9990~1.0000;平均回收率(n=6)为97.72%~99.12%,RSD为0.61%~2.01%。结论该方法专属性强、灵敏高、重复性好,适用于杞菊地黄胶囊中莫诺苷、绿原酸、马钱苷、木犀草苷、丹皮酚、23-乙酰泽泻醇C和23-乙酰泽泻醇B 7种有效成分的同时测定。Objective To establish an UPLC-MS/MS method for simultaneous determination of seven effective components(morroniside,chlorogenic acid,loganin,luteolin-7-O-glucoside,paeonol,23-acetate alisol C,23-acetate alisol B)in Qiju Dihuang Capsule.Methods The ACQUITY UPLC■HSS T3(100 mm×2.1 mm,1.8μm)column was adopted with mobile phase consisted of acetonitrile-0.1%-formic acid with gradient elution at the flow rate of 0.3 mL·min^(-1).Electrospray ionization(ESI)and multiple reactive ion monitoring(MRM)modes were used to determine the seven active components.Results The contents of morroniside,chlorogenic acid,loganin,luteolin-7-O-glucoside,paeonol,23-acetate alisol C,23-acetate alisol B in the samples per capsule were 0.28~0.35,0.08~0.11,0.30~0.48,0.21~0.48,0.62~0.76,0.04~0.08,0.15~0.32 mg.Correlation coefficient values of the 7 active components were among 0.9990~1.0000;the average recovery rates were among 97.72%~99.12%(n=6),and the RSD were among 0.61%~2.01%.Conclusion The method established is specific,sensitive and reproducible,which is suitable for the determination of seven effective components(morroniside,chlorogenic acid,loganin,luteolin-7-O-glucoside,paeonol,23-acetate alisol C,23-acetate alisol B)in Qiju Dihuang Capsule.

关 键 词：杞菊地黄胶囊 超高效液相色谱-串联质谱 莫诺苷 绿原酸 马钱苷 木犀草苷 丹皮酚 23-乙酰泽泻醇C 23-乙酰泽泻醇B 

分 类 号：R284.1[医药卫生—中药学]