E1A激活基因阻遏子基因对血管紧张素Ⅱ刺激后小鼠原代心肌细胞凋亡影响  

Effect of cellular repressor of E1A-stimulated genes on the apoptosis of mouse primary cardiomyocytes induced by angiotensinⅡ

作　　者：刘丹 宋海旭 闫承慧 刘艳霞 LIU Dan;SONG Hai-xu;YAN Cheng-hui;LIU Yan-xia(Department of Cardiology,General Hospital of Northern Theater Command,Shenyang 110016,China)

机构地区：[1]北部战区总医院心血管内科,辽宁沈阳110016

出　　处：《临床军医杂志》2021年第10期1113-1116,共4页Clinical Journal of Medical Officers

基　　金：国家自然科学基金面上项目(82070308);辽宁省优秀青年科学基金(2021-YQ-03);沈阳市科技计划项目(19-112-4-056);沈阳市科技计划项目(20-205-4-057)。

摘　　要：目的探讨E1A激活基因阻遏子基因(CREG)对血管紧张素Ⅱ(AngⅡ)刺激后小鼠原代心肌细胞凋亡的影响。方法将小鼠乳鼠原代心肌细胞培养后分为对照组与AngⅡ组(刺激24 h),采用荧光定量PCR和Western blot检测CREG基因mRNA和蛋白表达;将CREG过表达的腺病毒(adCREG)及其对照病毒(adcon)加入到小鼠原代心肌细胞上清后再给予AngⅡ刺激24 h后,Western blot检测凋亡相关指标Cleaved-caspase3、Bax和Bcl-2蛋白表达。结果对照组与AngⅡ组的CREG基因mRNA表达情况比较,差异无统计学意义(P>0.05);但AngⅡ组CREG蛋白水平明显低于对照组,差异有统计学意义(P<0.01)。adCREG组CREG蛋白表达明显高于adcon组,差异有统计学意义(P<0.01);但两组Cleaved-caspase3、Bax和Bcl-2蛋白表达比较,差异无统计学意义(P>0.05);adcon+AngⅡ组CREG和Bcl-2蛋白表达明显低于adcon组,而Cleaved-caspase3和Bax蛋白表达明显高于adcon组,差异均有统计学意义(P<0.01);adCREG+AngⅡ组Cleaved-caspase3和Bax蛋白表达明显低于adcon+AngⅡ组,而Bcl-2蛋白表达明显高于adcon+AngⅡ组,差异有统计学意义(P<0.01)。结论AngⅡ刺激后降低心肌细胞中CREG蛋白表达,CREG过表达后可显著抑制AngⅡ引起的心肌细胞凋亡。Objective To investigate the effect of cellular repressor of E1 A-stimulated genes(CREG)on the apoptosis of mouse primary cardiomyocytes induced by angiotensinⅡ(AngⅡ).Methods The primary myocardial cells of suckling mice were cultured and divided into control group and AngⅡgroup(stimulated for 24 hours).The mRNA and protein expression of CREG gene were detected by fluorescence quantitative PCR and Western blot.After CREG overexpressed adenovirus(adCREG)and its control virus(adcon)were added to the supernatant of mouse primary cardiomyocytes and then stimulated by AngⅡfor 24 hours,the expression of apoptosis related indicators Cleaved-caspase3,Bax and Bcl-2 proteins were detected by Western blot.Results There was no significant difference in the mRNA expression of CREG gene between control group and AngⅡgroup(P>0.05).However,CREG protein level in AngⅡgroup was lower than that in control group,the difference was statistically significant(P<0.01).The CREG protein expression in adCREG group was significantly higher than that in adcon group,and the difference was statistically significant(P<0.01).However,there was no significant difference in Cleaved-caspase3,Bax,and Bcl-2 protein expression between the adCREG group and adcon group(P>0.05).The expression of CREG and Bcl-2 protein in adcon+AngⅡgroup was significantly lower than that in adcon group,while the expression of Cleaved-caspase3 and Bax protein in adcon+AngⅡgroup was significantly higher than that in adcon group,and the difference was statistically significant(P<0.01).The expression of Cleaved-caspase3 and Bax protein in adCREG+AngⅡgroup was significantly lower than that in adcon+AngⅡgroup,while the expression of Bcl-2 protein was significantly higher than that in adcon+AngⅡgroup,and the difference was statistically significant(P<0.01).Conclusion The expression of CREG protein in cardiomyocytes was decreased after AngⅡstimulation,and the overexpression of CREG significantly inhibited the apoptosis of cardiomyocytes induced by AngⅡ.

关 键 词：E1A激活基因阻遏子基因 血管紧张素Ⅱ 心肌细胞 凋亡 

分 类 号：R542.2[医药卫生—心血管疾病]