团头鲂PGC1α分子特征及其对营养限制、高糖饲料和葡萄糖负荷的响应  

PGC1αmolecular characterization and its responsiveness to nutrient restriction,carbohydrate-enriched diets and glucose loadings in blunt snout bream(Megalobrama amblycephala)

作　　者：丁志荣 李向飞 刘文斌 谢帝芝 徐超 Jean-Jacques Yao Adjoumani DING Zhirong;LI Xiangfei;LIU Wenbin;XIE Dizhi;XU Chao;Jean-Jacques Yao Adjoumani(College of Marine Sciences of South China Agricultural University&Guangdong Laboratory for Lingnan Modern Agriculture,Guangzhou 510642,China;Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province,College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)

机构地区：[1]华南农业大学海洋学院,广东广州510642 [2]南京农业大学动物科技学院,江苏省水产动物营养重点实验室,江苏南京210095

出　　处：《水产学报》2021年第10期1657-1666,共10页Journal of Fisheries of China

基　　金：国家自然科学基金(31872576,32002361);国家现代农业产业技术体系(CARS-45-14)。

摘　　要：为探究过氧化物酶体增殖活化受体γ辅激活因子1α(peroxisome proliferator activated receptorγcoactivator 1α,PGC1α)基因在团头鲂糖代谢中的作用,实验从团头鲂肝脏中克隆获得了PGC1α基因的片段序列,并对其开展了生物信息学分析;同时研究了饥饿、高糖(糖水平:45%)饲料及葡萄糖负荷对团头鲂脑和肝脏PGC1α表达的影响。结果显示,获得的团头鲂PGC1α基因c DNA片段长为2566 bp,其中包含1404 bp的开放阅读框并编码467个氨基酸,与草鱼的同源性为96.79%;与正常投喂组相比,饥饿10 d组鱼脑和肝脏中PGC1α的mRNA水平显著升高,饥饿再投喂1 h后恢复至对照组的表达水平。饲喂高糖饲料(12周)后,与对照组相比,鱼脑和肝脏中PGC1α的mRNA水平显著降低。此外,葡萄糖负荷后,脑和肝脏中PGC1α的mRNA水平在2 h内显著降低至最小值,随后逐渐升高至基础水平。研究表明,PGC1α在团头鲂糖代谢过程中可能发挥重要作用。To explore the mechanism of peroxisome proliferator activated receptorγcoactivator 1α(PGC1α)in the glucose metabolism of blunt snout bream Megalobrama amblycephala,the partial cDNA of PGC1αwas cloned,and the transcriptional response of this gene to nutrient restriction,carbohydrate-enriched diets and glucose loadings were investigated.The partial cDNA was 2566 bp with an open reading frame of 1404 bp encoding 467 amino acids,and compared to Ctenopharyngodon idellus,it showed 96.79%homology.The mRNA levels of PGC1αin the brain and liver were significantly increased during 10 days of fasting,and then decreased to normal level after refeeding 1 h.The high-carbohydrate diet significantly decreased the mRNA levels of PGC1αin the brain and liver.In addition,the mRNA levels of PGC1αin the brain and liver both decreased significantly during the first 2 h,then returned to the basal value at 12 h.The results indicated that PGC1αplays an important role in glucose metabolism in M.amblycephala.The results obtained here will provide the theoretical foundation for completing the research of PGC1αfunctions involving the regulation of glucose homeostasis in fish.

关 键 词：团头鲂 过氧化物酶体增殖活化受体γ辅激活因子1α 糖代谢 基因克隆 基因表达 

分 类 号：S963.7[农业科学—水产养殖]