circ0092516通过靶向调控miR-337-3p/PTEN通路调控促进原发性腺性膀胱炎细胞的存活率和迁移率  

Effect of circ0092516 on the proliferation and migration potential of primary glandular cystitis cells through targeting miR-337-3p/PTEN pathway

作　　者：徐磊[1] 吴挺明 车宪平[1] 胡鑫明[1] 陈化磊[1] 许生 曹鹏 XU Lei;WU Tingming;CHE Xianping;HU Xinming;CHEN Hualei;XU Sheng;CAO Peng(Department of Urology,Second Affiliated Hospital of Hainan Medical College,Haikou,570311,China)

机构地区：[1]海南医学院第二附属医院泌尿外科一区,海口570311

出　　处：《临床泌尿外科杂志》2021年第11期881-888,共8页Journal of Clinical Urology

基　　金：海南省卫生健康行业科研项目(No:20A200406)。

摘　　要：目的:探讨circ0092516对腺性膀胱炎(CG)细胞存活和迁移的影响及其调控微小RNA(miR)-337-3p和同源性磷酸酶(PTEN)介导的机制。方法:从手术切除的10例CG样本中原代分离CG细胞(pCG),并从正常组织中分离正常对照组细胞(pCON)。qRT-PCR用于检测circ0092516和miR-337-3p在pCG和pCON细胞中的表达。荧光素酶报告基因测定法分别用于研究特定circ0092516和miR-337-3p、miR-337-3p和PTEN之间的相互作用。通过在体外转染circ0092516过表达质粒或者circ0092516沉默siRNA研究对pCG细胞存活率和迁移的影响。蛋白质印迹检测PTEN的表达。通过transwell检测细胞迁移和CCK-8检测细胞存活率变化。结果:circ0092516在pCG细胞中的表达高于pCON细胞(t=9.121,P<0.05)。沉默circ0092516在体外抑制细胞存活率和迁移(t=7.612、11.455,P<0.05)。荧光素酶报告基因实验观察到circ0092516直接与miR-337-3p相互作用,并抑制miR-337-3p的表达,促进miR-337-3p的靶基因PTEN的表达,从而促进pCG细胞的存活率和迁移率。结论:circ0092516通过调控miR-337-3p/PTEN轴促进CG细胞的存活率和迁移,是CG的治疗提供潜在的生物标志物和治疗靶标。Objective: To explore the role of circ0092516 in cystitis glandularis(CG) and its molecular mechanism of regulating microRNA(miR)-337-3 p and phosphatase and tensin homologue deleted on chromosome 10(PTEN) to mediate the proliferation and migration of CG cells. Methods: Primary CG cells(pCG) from CG samples of 10 CG patients were isolated, and control cells(pCON) from normal tissues were isolated. QRT-PCR was used to detect the expression of circ0092516 and miR-337-3 p in pCG and pCON cells. The luciferase reporter gene assay was used to study the interaction between specific circ0092516 and miR-337-3 p, miR-337-3 p and PTEN, respectively. The effect of circ0092516 on the proliferation and migration of pCG cells was studied by plasmid transfection in vitro. The expression of PTEN was detected by Western blot. Transwell was used to detect cell migration and CCK-8 to detect cell proliferation changes. Results: The expression of circ0092516 in pCG cells was higher than that in pCON cells(t=9.121, P<0.05). Silencing circ0092516 inhibited cell proliferation and migration in vitro(t=7.612, 11.455, P<0.05). In terms of mechanism, circ0092516 can directly interact with miR-337-3 p, and circ0092516 regulated the expression of miR-337-3 p’s target gene PTEN, thereby regulating the development of pCG. Conclusion: Circ0092516 promotes the proliferation and migration of CG cells by regulating the miR-337-3 p/PTEN axis, so it is a potential biomarker and therapeutic target for the treatment of CG.

关 键 词：circ0092516 同源性磷酸酶 原发性腺性膀胱炎 细胞存活率和迁移 

分 类 号：R694[医药卫生—泌尿科学]