ABPPk对施万细胞血清剥夺损伤的保护作用机制研究  

Protective mechanism of ABPPk on serum deprivation injury in Schwann cells

作　　者：仲晶飞 周家名 杨晋娴 李枚原[2] ZHONG Jingfei;ZHOU Jiaming;YANG Jinxian;LI Meiyuan(Medical Department of Nantong University Xinglin College;Jiangsu Key Laboratory of Neuroregeneration,Nantong University,Nantong 226001)

机构地区：[1]南通大学杏林学院医学部 [2]南通大学江苏省神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2021年第6期499-501,F0002,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金资助项目(81901256);江苏省高等学校自然科学研究面上项目(19KJD310001);南通大学杏林学院自然科学基金资助项目(2016K135);南通市科技计划项目(JC2020104)。

摘　　要：目的:探讨牛膝多肽k(Achyranthes bidentata polypeptides k,ABPPk)对血清剥夺引起的施万细胞凋亡的保护作用机制。方法:通过转录组测序及生物信息学方法分析ABPPk对施万细胞血清剥夺损伤的保护作用机制。结果:转录组测序及生物信息学分析结果显示,ABPPk组主要参与正向调节粒细胞分化、Rho蛋白信号转导、肌动蛋白细胞骨架、细胞增殖、蛋白质泛素化等生物学过程,Rhog、Pak2、Yes1、Mef2c、Dock7等基因参与其中;神经生长因子(nerve growth factor,NGF)组主要参与囊泡介导的运输、胞吞作用、细胞形态调节、钙离子稳态调节等生物学过程,Kalrn、Mapk6、Sgk1、Cdc42bpb、Cnot6等基因参与其中。结论:ABPPk和NGF对施万细胞血清剥夺的保护作用机制有所不同,主要调控分子也不同。Objective:To explore the protective mechanism of Achyranthes bidentata polypeptides k(ABPPk)on Schwann cells apoptosis caused by serum deprivation.Methods:Transcriptome sequencing and bioinformatic methods were used to analyze the protective mechanism of ABPPk on serum deprivation injury in Schwann cells.Results:Transcriptome sequencing and bioinformatics analysis results showed that the ABPPk group was mainly involved in the positive regulation of granulocyte differentiation,Rho protein signal transduction,actin cytoskeleton organization,cell proliferation,protein ubiquitination and other biological processes.Pak2,Yes1,Mef2c,Dock7 and other genes were involved.Nerve growth factor(NGF)group was mainly involved in vesicle-mediated transport,endocytosis,regulation of cell shape,cellular calcium ion homeostasis and other biological processes.Kalrn,Mapk6,Sgk1,Cdc42bpb,Cnot6 and other genes were involved.Conclusion:The protective mechanism of ABPPk and NGF against serum deprivation of Schwann cells is different,and the main regulatory molecules are also different.

关 键 词：牛膝多肽k 施万细胞 血清剥夺损伤 生物信息学 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]