HPLC同时测定斑秃丸中11种活性组分  

Simultaneous Determination of 11 Kinds of Active Components in Bantu Pills by HPLC

作　　者：霍甜甜 李宜鲜[1] HUO Tian-tian;LI Yi-xian(Key Laboratory of Quality Control of Chinese Medicinal Materials and Decoction Pieces,State Drug Administration,Henan Institute for Food and Drug Control,Zhengzhou 450009,China)

机构地区：[1]河南省食品药品检验所国家药品监督管理局中药材及饮片质量控制重点实验室,河南郑州450009

出　　处：《食品与药品》2021年第6期524-529,共6页Food and Drug

摘　　要：目的建立高效液相色谱(HPLC)同时测定斑秃丸中芍药苷、毛蕊花糖苷、二苯乙烯苷、阿魏酸、丹酚酸B、五味子醇甲、大黄素、异欧前胡素、五味子甲素、丹参酮IIA和五味子乙素含量的方法。方法采用HPLC,Venusil MP C18色谱柱(4.6 mm×250 mm,5μm);以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,流速为1.0 ml/min;进样量为10μl;检测波长为220 nm;柱温为35℃。结果芍药苷在51.98~1559.46 ng(r=1.0000),毛蕊花糖苷在21.84~655.20 ng(r=1.0000),二苯乙烯苷在103.60~3108.00 ng(r=0.9999),阿魏酸在6.79~203.67 ng(r=1.0000),丹酚酸B在79.80~2393.91 ng(r=1.0000),五味子醇甲在20.94~628.20 ng(r=1.0000),大黄素在7.13~213.81 ng(r=1.0000),异欧前胡素在18.16~544.80 ng(r=1.0000),五味子甲素在6.71~201.15 ng(r=1.0000),丹参酮IIA在3.25~97.44 ng(r=0.9999),五味子乙素在7.96~238.80 ng(r=1.0000)进样量范围内与峰面积呈良好线性关系;平均加样回收率分别为97.64%,102.61%,96.95%,98.23%,95.64%,99.53%,97.90%,99.02%,103.55%,102.31%,100.24%,RSD均<5%(n=6)。结论本方法专属性强,灵敏度高,可有效控制斑秃丸的质量。Objective To develop an HPLC method for the determination of paeoniflorin, verbascoside, stilbene glucoside,ferulic acid, salvianolic acid B, schisandrin, emodin, isoimperatorin, deoxyschizandrin, tanshinone IIA and schisandrin B in Bantu Pills at the same time. Methods The chromatographic separation was achieved on a Venusil MP C18 column(4.6 mm×250 mm, 5 μm), using acetonitrile-0.1 % phosphoric acid solution at the flow rate of 1.0 ml/min as mobile phase for gradient elution. The injection amount was 10 μl, the detection wavelength was set at 220 nm, and column temperature was 35 ℃. Results Paeoniflorin, verbascoside, stilbene glucoside, ferulic acid, salvianolic acid B, schisandrin, emodin,isoimperatorin, deoxyschizandrin, tanshinone IIA and schisandrin B showed good linear relationships between injection amount and peak area at the ranges of 51.98-1559.46 ng(r=1.0000), 21.84-655.20 ng(r=1.0000), 103.60-3108.00 ng(r=0.9999), 6.79-203.67 ng(r=1.0000), 79.80-2393.91 ng(r=1.0000), 20.94-628.20 ng(r=1.0000), 7.13-213.81 ng(r=1.0000), 18.16-544.80 ng(r=1.0000), 6.71-201.15 ng(r=1.0000), 3.25-97.44 ng(r=0.9999) and 7.96-238.80 ng(r=1.0000). The average recoveries were 97.64 %, 102.61 %, 96.95 %, 98.23 %, 95.64 %, 99.53 %, 97.90 %, 99.02 %,103.55 %, 102.31 % and 100.24 %, and RSDs were<5 %(n=6). Conclusion The established analytical method is of strong specificity and high sensitivity, and can be used efficiently in the quality control of Bantu Pills.

关 键 词：高效液相色谱 斑秃丸 活性组分 含量测定 

分 类 号：R917[医药卫生—药物分析学]