生物炭促进硫诱导土著硫杆菌调控土壤铅形态  

Biochar promotes the regulation of fraction distribution of soil lead by sulfur-induced indigenous Thiobacillus

作　　者：叶俊沛 陈楠 张盼月[2] 蔡雅静 杨卓越 常建宁 朱桂芬 YE Junpei;CHEN Nan;ZHANG Panyue;CAI Yajing;YANG Zhuoyue;CHANG Jianning;ZHU Guifen(School of Environment,Henan Normal University,Xinxiang 453007,China;College of Environmental Science and Engineering,Beijing Forestry University,Beijing 100083,China;Key Laboratory of Yellow River and Huai River Water Environment and Pollution Control,Ministry of Education,Xinxiang 453007,China;Henan Key Laboratory for Environmental Pollution Control,Xinxiang 453007,China)

机构地区：[1]河南师范大学环境学院,新乡453007 [2]北京林业大学环境科学与工程学院,北京100083 [3]黄淮水环境与污染防治教育部重点实验室,新乡453007 [4]河南省环境污染控制重点实验室,新乡453007

出　　处：《环境工程学报》2021年第10期3270-3278,共9页Chinese Journal of Environmental Engineering

基　　金：河南师范大学博士科研启动项目(5101219170148);河南师范大学优秀科技创新团队(2021TD06);河南省高校科技创新团队(20IRTSTHN011);河南省科技厅科技攻关计划项目(212102310528)。

摘　　要：外源硫杆菌可以促进植物修复重金属污染土壤,然而硫杆菌对土壤中铅的活化作用机理尚不明确。通过土培实验,研究了单质硫和生物炭对土著硫杆菌的诱导作用和对土壤铅形态的作用机理。结果表明,单质硫能够诱导并富集土壤中的土著硫杆菌,相对丰度从0.02%增加至6.99%。生物炭促进了诱导作用,添加生物炭和单质硫,硫杆菌相对丰度增加至13.33%。同时添加3%(质量分数)生物炭和0.5%(质量分数)单质硫培养60 d后,土壤pH从7.83降低至6.64, Eh先降低至230 mV,后逐渐增加至299 mV;有机碳从19.40 g·kg^(-1)增加至28.29 g·kg^(-1);土壤中铅由可还原态向酸可溶态、可氧化态和残渣态转变,可还原态铅减少了20.13%,酸可溶态、可氧化态和残渣态分别增加了53.76%、35.29%和69.40%。单质硫和生物炭能够富集土著硫杆菌,提高铅的生物有效性,同时对部分铅起到钝化固定作用。本研究可为提高土壤中铅的生物有效性以促进植物修复铅污染土壤提供参考。The promotion of exotic Thiobacillus on phytoremediation has attracted in recent years, however,the mobilization mechanism of Thiobacillus on soil lead is still unclear. The mechanism of sulfur and biochar on the induction of indigenous Thiobacillus and the distribution of soil lead content were studied through soil culture experiments. The relative abundance of Thiobacillus in soil was increased from 0.02% to 6.99%, which was inducted by sulfur in soil. The induction was promoted by biochar. The relative abundance of Thiobacillus was increased to 13.33% with biochar addition. With a 3%(mass fraction) of biochar and 0.5%(mass fraction)of sulfur addition in a 60 d pot experiment, the soil pH was decreased from 7.83 to 6.64. The soil Eh was decreased to 230 mV directly at the beginning of experiment, and increased to 299 mV after 60 d. The soil organic carbon was increased from 19.40 g·kg^(-1)to 28.29 g·kg^(-1). 20.13% of reducible Pb species was transformed to acid extractable, oxidizable and residual Pb species, which was increased by 53.76%, 35.29% and 69.40%, respectively. The combined of biochar and sulfur can promote the bioavailability, and immobilize Pb in soil at same time. This study can provide an approach for regulating the Pb bio-availability for phytoremediation of Pb-contaminated soil.

关 键 词：铅污染土壤 硫杆菌 生物炭 重金属形态 生物有效性 

分 类 号：X53[环境科学与工程—环境工程]