七氟醚麻醉对胃癌大鼠认知功能的影响及机制研究  

Study on effect and mechanism of sevoflurane anesthesia on cognitive function in rats with gastric cancer

作　　者：程亮亮[1] 田毅[1] 黄运伯[1] 魏晓[1] CHENG Liangliang;TIAN Yi;HUANG Yunbo;WEI Xiao(Department of Anesthesiology,Affiliated Haikou Hospital of Xiangya School of Medicine,Central South University,Haikou,Hainan 570208 China)

机构地区：[1]中南大学湘雅医学院附属海口医院麻醉科,海口570208

出　　处：《重庆医学》2021年第23期3994-3998,共5页Chongqing medicine

基　　金：国家自然科学基金项目(81660195);海南省卫生健康行业科研项目(20A200157)。

摘　　要：目的探讨七氟醚麻醉对胃癌大鼠认知功能的影响,并分析其可能作用机制。方法选取50只健康雄性SD大鼠,根据随机数字表法分为A、B、C、D、E 5组,每组10只。除A组外,其余4组均采用肌氨酸乙酯亚硝胺、亚硝酸钠致癌剂灌服法制备胃癌大鼠模型,建模成功大鼠纳入实验。A组给予丙泊酚麻醉2 h;B组在丙泊酚麻醉同时行腹部探查术;C、D、E组分别在1%、2%、3%七氟醚吸入麻醉同时行腹部探查术。Morris水迷宫实验评估各组大鼠学习、记忆能力,酶联免疫吸附试验(ELISA)检测各组大鼠海马组织超氧化物歧化酶(SOD)、丙二醛、淀粉样前体蛋白(APP);Western blot法检测各组大鼠海马组织胶质原纤维酸性蛋白(GFAP)、肿瘤坏死因子-α(TNF-α)、半胱氨酸天冬氨酸蛋白酶-9(caspase-9)表达水平。结果与水迷宫测试定位航行试验第1天比较,第4天时5组大鼠逃避潜伏期、目标象限游泳距离均明显缩短(P<0.05);A、B、C、D、E组大鼠组间逃避潜伏期、目标象限游泳距离呈逐渐延长趋势(P<0.05),穿越平台次数呈逐渐减少趋势(P<0.05)。A、B、C、D、E组大鼠组间SOD水平呈逐渐下降趋势(P<0.05),丙二醛、APP水平及GFAP、TNF-α、caspase-9相对表达水平呈逐渐增高趋势(P<0.05)。结论七氟醚麻醉可能经诱导应激反应、炎性反应及促进细胞凋亡,并参与胃癌大鼠认知功能障碍发生发展过程。Objective To explore the effect of sevoflurane anesthesia on the cognitive function of rats with gastric cancer,and to analyze its possible action mechanism.Methods Fifty healthy male SD rats were selected and randomly divided into the group A,B,C,D and E according to the random number table method,10 cases in each group.Except the group A,the other 4 groups were prepared the gastric cancer rat models by oral administration of sarcosine ethyl chloride and sodium nitrite carcinogen.The successful modeling rats were included in the experiment.The group A was given propofol anesthesia for 2,the group B simultaneously conducted propofol anesthesia and abdominal exploration;the group C,D and E conducted 1%,2%and 3%sevoflurane inhalation anesthesia respectively,and at the same time conducted the abdominal exploration.The learning and memory abilities were evaluated by the Morris water maze experiment.The levels of superoxide dismutase(SOD),malondialdehyde,amyloid precursor protein(APP)in the hippocampus tissue were detected by the enzyme-linked immunosorbent test(ELISA).The expressions of glial fibrillary acidic protein(GFAP),tumor necrosis factor-α(TNF-α)and cystein-asparate protease 9(caspase-9)were detected by Western blot.Results Compared with the water maze test on 1 d of the navigation test,the latency and the swimming distance of the target quadrant on 4 d in 5 groups were significantly shortened(P<0.05),the escape latency,target quadrant swimming distance in the rats of the A,B,C,D and E groups showed a gradual extension trend(P<0.05),and the number of crossing platforms showed a gradual decrease trend(P<0.05).The SOD level in the rats of the group A,B,C,D and E showed a gradual decrease trend(P<0.05),and the levels of malondialdehyde and APP,and the relative expression amounts of GFAP,TNF-αand caspase-9 showed a gradual increase trend(P<0.05).Conclusion Sevoflurane anesthesia may induce the stress response,inflammatory response and promoting cell apoptosis,and participates in the occurrence and development

关 键 词：胃癌 七氟醚 应激反应 认知功能障碍 大鼠 

分 类 号：R332[医药卫生—人体生理学]