绝经后女性LRP5与LRP6基因位点的多态性及交互作用与骨量的异常关系  

Study on the relationship between gene polymorphisms and interactions in LRP5 and LRP6 and abnormal bone mass in postmenopausal women

作　　者：邵晗 李思源[1] 高群 李军[2] SHAO Han;LI Si-yuan;GAO Qun;LI Jun(Shihezi University School of Medicine,Shihezi 832000,Xinjiang,China;Department of Endocrinology,the First Afiliated Hospital,School of Medicine,Shihezi University,Shihezi 832000,Xinjiang,China)

机构地区：[1]新疆石河子大学医学院,石河子832000 [2]新疆石河子大学医学院第一附属医院内分泌代谢科,石河子832000

出　　处：《医学研究生学报》2021年第11期1171-1176,共6页Journal of Medical Postgraduates

基　　金：新疆生产建设兵团区域创新引导计划(2018BB040);石河子大学成果转化项目与技术推广项目(CGZH201911)。

摘　　要：目的LRP5基因位点的多态性与绝经后2型糖尿病(T2DM)女性患者骨密度降低有关。文中探讨绝经后女性L.RP5与LRP6基因位点的多态性及交互作用与骨密度的关系。方法选取2018年12月-2019年12月期间,石河子大学医学院第一-附属医院272例绝经后女性临床资料。根据患者骨密度测量结果分为骨量正常组(对照组)、骨量异常组(ABM组),收集分析各组患者基线资料,测定骨代谢指标。采用DEXA测定腰椎1-4(L.4)和股骨颈(FN)的BMID。采用飞行时间质谱法(MALDI-TOF MS法)测定LRP5基因rs2306862、rs41494349位点和LRP6基因rs10743980、rs2302685位点多态性及基因频率分布。结果Logistic回归分析显示,LRP5基因rs2306862位点CT、CT/TT基因型患ABM的风险高于CC基因型(OR=2.353,95%C1=1.039~6.186;0R=2.434,95%CI=1.071,5.531;P<0.05);LRP6基因rs2302685位点TC基因型患ABM的风险高于TT基因型(OR=2.951,95%Cl=1.030~8.457,P<0.05)。余各SNP位点未见明显异常(P>0.05)。rs2306862&rs 10743980、rs414943498rs2302685&rs10743980位点的多态性与ABM的发生存在协同作用,为ABM发生的危险性因素(P<0.05);rs2306862、rs2302685、rs41494349&rs2302685&rs10743980位点的多态性&年龄与ABM的发生存在协同作用,为ABM发生的危险性因素(P<0.05);各位点基因多态性&绝经年限与ABM的发生存在交互作用(P>0.05)。结论新疆绝经后女性LRP5-s2306862和LRP6-rs2302685多态性.基因-基因、基因-年龄的交互作用与ABM的风险增高有关。Objective Previous studies have found that polymorphism of LRP5 is associated with decreased bone density in postmenopausal women with type 2 diabetes mellitus(T2DM).This paper aims to explore the relationship between gene polymorphism and interactions in LRP5 and LRP6 and abnormal bone mass in postmeno-pausal women.Methods Clinical data of 272 postmenopausal women from the First Afiliated Hospital of Shihezi University Medical College from December 2018 to December 2019 were selected.Ac-cording to BMD measurement results,patients were divided into nor-mal group(control group)and abnormal group(ABM group).Base-line data of patients in each group were collected and analyzed,and.bone metabolism indexes were determined.BMD of lumbar 14(L 14)and femoral neck(FN)were measured by DEXA.The polymorphism and gene frequency distribution of rs2306862 and s41494349 of LRP 5 and rs10743980 and rs2302685 of LRP 6 were determined by MALDI TOF MS.Results Logistie regression analysis showed that the risk of ABM was higher in CT and CT/TT genotype of LRP5 rs2306862 than that in CC genotype(OR=2.353,95%CI=1.039~6.186;OR=2.434,95%CI=1.071,5.531;P<0.05);The risk of ABM was higher in TC genotype of LRP6 rs2302685 than that in TT genotype(OR=2.951,95%CI=1.030-8.457,P<0.05).No significant abnormality was observed in the remaining SNP loci(P>0.05).r82306862&rs10743980,rs41494349&rs2302685&rs 10743980 polymorphism had synergistic ffect on and were risk factors for the ocurrence of ABM(P<0.05).rs2306862,rs2302685,rs414943498&rs2302685&rs10743980 polymorphism and age had synergis-tie effect on and was a risk factor for the occurrence of ABM(P<0.05).There was an interaction betwen ABMI occurrence and gene polymorphism at each locus and duration of menopause(P>0.05).Conclusion The interaction of LRPS rs2306862 and LRP6 rs2302685 poly morphism,gene-gene and gene-age in postmenopausal women in Xinjiang was associated with the increased risk of ABM.

关 键 词：低密度脂蛋白受体相关蛋白5 低密度脂蛋白受体相关蛋白6 基因多态性 骨质疏松 

分 类 号：R68[医药卫生—骨科学]