从大约克猪乳中提取DNA用于mtDNA D-loop序列分析  

Extraction of DNA from milk of Yorkshire pigs for mtDNA D-loop sequence analysis

作　　者：张建梅 黄卫平 金素钰[1] 邹桐 黄林[1] 郑玉才[1] ZHANG Jian-mei;HUANG Wei-ping;JIN Su-yu;ZOU Tong;HUANG Lin;ZHENG Yu-cai(School of Animal and Veterinary Sciences,Southwest Minzu University,Chengdu 610041,China;Sichuan Liangshan Prefecture Institute of Animal Science,Xichang 615042,China)

机构地区：[1]西南民族大学畜牧兽医学院,四川成都610041 [2]四川省凉山彝族自治州畜牧科学研究所,四川西昌615042

出　　处：《西南民族大学学报（自然科学版）》2021年第6期568-573,共6页Journal of Southwest Minzu University(Natural Science Edition)

基　　金：四川省科技扶贫(产业类)项目(2018NFP0160)。

摘　　要：为阐明一个大约克猪群体的遗传多样性,本实验用Chelex-100方法从来自四川省凉山州的30头大约克猪乳中提取基因组DNA,并用PCR方法扩增mtDNA D-loop区部分序列.结果显示,通过优化PCR循环数等参数,可以扩增出乳源基因组DNA的mtDNA D-loop区,产物可直接测序,获得364 bp的序列.30个大约克猪mtDNA D-loop区单倍型多样度(Hd)为0.618;核苷酸多样度(Pi)为0.00860,显示了较丰富的遗传多样性;检测到11个多态位点,界定了7种单倍型,其中具有Hap3单倍型的个体比例占50%以上.结果表明,乳源基因组DNA可用于mtDNA D-loop区的PCR扩增及直接测序,试验大约克猪母系遗传多样性较高.In order to assay the genetic diversity of a Yorkshire pig population,genomic DNA was extracted from 30 milk samples of Yorkshire pigs using Chelex-100 method,and partial mtDNA D-loop region was amplified by PCR and sequenced.The results showed that the mtDNA D-loop region could be amplified successfully using milk derived genomic DNA,and the PCR products could be directly sequenced,resulting in a 364 bp sequence.The haplotype diversity(Hd)and nucleotide diversity(Pi)of 30 Yorkshire pigs mtDNA D-loop were 0.618 and 0.00860,respectively,indicating a relevant high genetic diversity of this population.Eleven polymorphic sites,defining 7 haplotypes were detected,and more than 50%of the individuals carried Hap3 haplotype.The present results suggested that milk derived genomic DNA could be used for PCR amplification of the mtDNA D-loop region and subsequent direct sequencing,and the maternal genetic diversity of the experimental Yorkshire pigswas relatively abundant.

关 键 词：大约克猪 乳 mtDNA D-loop 遗传多样性 

分 类 号：S813[农业科学—畜牧学] S828[农业科学—畜牧兽医]