知黄溃疡合剂对复发性口腔黏膜溃疡大鼠创面修复的作用及对MCP-1/CCR2/NF-κB通路的影响  

Effect of Zhihuang Ulcer Mixture(知黄溃疡合剂)on Wound Healing and MCP-1/CCR2/NF-κB Pathway in Rats with Recurrent Oral Mucosal Ulcer

作　　者：耿乾书 姚娜 王雯 李庆隆[1] GENG Qian-shu;YAO Na;WANG Wen;LI Qing-long(Tangshan Union Medical College Hospital,Tangshan Hebei 063000,China)

机构地区：[1]唐山市协和医院,河北唐山063000

出　　处：《中医药导报》2021年第11期50-55,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：河北省卫生和计划生育委员会科研基金项目(20171310)。

摘　　要：目的:探讨知黄溃疡合剂对复发性口腔黏膜溃疡模型大鼠创面修复的作用及对单核细胞趋化蛋白-1(MCP-1)/趋化因子受体-2(CCR2)/核因子-κB(NF-κB)通路的影响。方法:55只SD大鼠随机选取10只作为对照组,其余45只大鼠建立复发性口腔黏膜溃疡大鼠模型,40只造模成功大鼠随机分为模型组、左旋咪唑组、知黄溃疡合剂低剂量组、知黄溃疡合剂高剂量组,每组10只。左旋咪唑组、知黄溃疡合剂低剂量组、知黄溃疡合剂高剂量组按10 mL/kg灌胃给药,对照组和模型组大鼠则予以等体积生理盐水灌胃,连续给药20 d。观察大鼠口腔溃疡情况变化;ELISA法测定大鼠溃疡黏膜组织中炎症因子水平;流式细胞仪检测大鼠外周血T淋巴细胞亚群;HE染色观察大鼠口腔黏膜组织病理;Western blotting测定大鼠溃疡黏膜组织中MCP-1、CCR2、NF-κB、p-NF-κB p65蛋白表达水平。结果:模型组、知黄溃疡合剂低剂量组、知黄溃疡合剂高剂量组、左旋咪唑组大鼠溃疡数目依次减少,溃疡评分依次降低,γ干扰素(IFN-γ)、白细胞介素17(IL-17)依次降低,CD4;、CD4;/CD8;依次升高,CD8;依次降低(P<0.05)。模型组大鼠口腔黏膜组织破溃,可见大量空泡变性,大量炎症细胞浸润;左旋咪唑组、知黄溃疡合剂高剂量组明显改善,知黄溃疡合剂低剂量组有所改善。模型组、知黄溃疡合剂低剂量组、知黄溃疡合剂高剂量组、左旋咪唑组大鼠溃疡黏膜组织中MCP-1、CCR2蛋白相对表达量及p-NF-κB p65/NF-κB依次降低(P<0.05)。结论:知黄溃疡合剂可能通过抑制MCP-1/CCR2/NF-κB通路,发挥对复发性口腔黏膜溃疡大鼠创面的修复作用。Objective: To investigate the effect of Zhihuang ulcer mixture on wound healing and monocyte chemoattractant protein-1(MCP-1)/chemokine receptor-2(CCR2)/nuclear factor kappa B(NF-κB) pathway in rats with recurrent oral mucosal ulcer. Methods: 10 of 55 SD rats were randomly selected as the control group, and the other 45 rats were used to establish the model of recurrent oral mucosal ulcer. 40 successful rats were randomly divided into model group, levamisole group, low-dose Zhihuang ulcer mixture group and high-dose Zhihuang ulcer mixture group, with 10 rats in each group. The levamisole group, low-dose group and high-dose group were administered by gavage at the rate of 10 ml/kg, while rats in the control group and model group were administered by gavage with equal volume of normal saline, all rats were treated for 20 days. The changes of oral ulcer were observed;The levels of inflammatory factors in rat ulcer mucosa were measured by ELISA.T lymphocyte subsets in rat peripheral blood were detected by flow cytometry. He staining was used to observe the histopathology of rat oral mucosa. The expression level of MCP-1, CCR2, NF-κB and p-NF-κB p65 protein were measured by Western blotting. Results: In the model group, low-dose group, high-dose group and levamisole group, the number and score of ulcers decreased gradually, interferon gamma(IFN-γ), interleukin 17(IL-17) decreased gradually, CD4;, CD4;/CD8;increased gradually, and CD8;decreased gradually(P<0.05). The oral mucosal tissue of the model group was ruptured, with a large number of vacuolar degeneration and inflammatory cell infiltration. Levamisole group and high dose group were significantly improved, and low dose group was improved. In the model group, low-dose group, high-dose group and levamisole group, the relative expression of MCP-1 protein, CCR2 protein, and p-NF-κB p65/NF-κB p65 decreased gradually(P<0.05). Conclusion: Zhihuang Ulcer Mixture may play a role in wound repair of rats with recurrent oral mucosal ulcer by inhibiting MCP-1/CCR2/p-NF

关 键 词：复发性口腔黏膜溃疡 知黄溃疡合剂 单核细胞趋化蛋白-1 趋化因子受体-2 核因子-ΚB 大鼠 

分 类 号：R285.5[医药卫生—中药学]