利用分子标记辅助选择技术聚合多基因改良两系不育系创5S的褐飞虱抗性  

Pyramiding Multiple Resistance Genes to Brown Planthopper by Marker-assisted Selection to Improve Brown Planthopper Resistance of Rice PTGMS Line Chuang 5S

作　　者：李晓雪 鄢卫东 彭田伟 欧阳兆彭 雷东阳[1,3] LI Xiao-xue;YAN Wei-dong;PENG Tian-wei;OUYANG Zhao-peng;LEI Dong-yang(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China;Seed Administration station,Bureau of Agricultural and Rural Affairs of Zhongfang County,Huaihua,Hunan 418000,China;Hunan Liang'an Sci-Tech Co.Ltd.,Shaoyang,Hunan 422008,China)

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]中方县农业农村局种子管理站,湖南怀化418000 [3]湖南粮安科技股份有限公司,湖南邵阳422008

出　　处：《杂交水稻》2021年第6期84-88,共5页Hybrid Rice

基　　金：长沙市科技计划项目(kq197043);湖南省大学生创新创业训练计划项目(2423)。

摘　　要：利用创5S为受体亲本的褐飞虱抗性改良中间材料7CS18[含Bph18(t)基因]和7CS10[含Bph20(t)、Bph21(t)基因]进行杂交,并在后代利用分子标记技术结合田间农艺性状筛选,获得了聚合Bph18(t)、Bph20(t)和Bph21(t)等3个纯合稻飞虱抗性基因的水稻两系不育系改良株系9S522。苗期抗性鉴定表明,创5S、7CS10和7CS18对褐飞虱抗性等级分别为"感""抗"和"中抗",改良株系9S522对褐飞虱抗性等级为"抗";改良株系9S522农艺性状与创5S基本保持一致。Using the Chuang 5S-derived improved intermediate materials 7CS18[bearing Bph18(t)]and 7 CS10[bearing Bph20(t)and Bph21(t)]with brown planthopper(BPH)resistance to make a cross followed by field selection of agronomic characters and marker-assisted selection of BPH resistance genes,a new photoperiod-and thermo-sensitive genic male sterile(PTGMS)rice line 9 S522 with three homogenous BPH resistant genes of Bph18(t),Bph20(t)and Bph21(t)was obtained.The identification of resistance at seedling stage showed that the level of BPH resistance was susceptible,resistant and moderately resistant in Chuang 5S,7CS10 and 7CS18,respectively,and the improved line 9S522 with polygenes of BPH was resistance to BPH and nearly keep same agronomic characters to Chuang 5S.

关 键 词：杂交水稻 两系不育系 创5S 褐飞虱 抗性基因 基因聚合 分子标记 

分 类 号：S511.01[农业科学—作物学] Q75[生物学—分子生物学]