The INO80 chromatin remodeling complex promotes thermo-morphogenesis by connecting H2A.Z eviction and active transcription in Arabidopsis.  

作　　者：Xue Mande Zhang Huairen Zhao Fengyue Zhao Ting Li Hui Jiang Danhua 

机构地区：[1]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,The Innovative Academy for Seed Design,Chinese Academy of Sciences,Beijing,China [2]University of Chinese Academy of Sciences,Beijing,China

出　　处：《Molecular Plant》2021年第11期1799-1813,共15页分子植物（英文版）

基　　金：This work was supported by the National Key R&D Program of China(2019YFA0903903);the Strategic Priority Research Program of the Chinese Academy of Sciences(Precision Seed Design and Breeding,XDA24020303);the National Natural Science Foundation of China(31970527).

摘　　要：Global warming imposes a major threat to plant growth and crop production. In some plants including Arabidopsis thaliana, elevated temperatures induce a series of morphological and developmental adjustments, termed thermomorphogenesis to facilitate plant cooling under high-temperature conditions. Plant thermal response is suppressed by histone variant H2A.Z. At warm temperatures, H2A.Z is evicted from nucleosomes at thermo-responsive genes, resulting in their expression changes. However, the mechanisms that regulate H2A.Z eviction and subsequent transcription changes are largely unknown. Here, we show that the INO80 chromatin remodeling complex (INO80-C) promotes thermomorphogenesis and activates the expression of thermo-responsive and auxin-related genes. INO80-C associates with PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), a potent regulator in thermomorphogenesis, and mediates temperature-induced H2A.Z eviction at PIF4 targets. Moreover, INO80-C directly interacts with COMPASS-like and transcription elongation factors to promote active histone modification Histone H3 lysine 4 trimethylation (H3K4me3) and RNA Polymerase II (RNA Pol II) elongation, leading to the thermal induction of transcription. Notably, transcription elongation factors SPT4 and SPT5 are required for the H2A.Z eviction at PIF4 targets, suggesting the cooperation of INO80-C and transcription elongation in H2A.Z removal. Our results demonstrate that the (PIF4)-(INO80-C)-(COMPASS-like)-(transcription elongator) module controls plant thermal response, and establish a link between H2A.Z eviction and active transcription.

关 键 词：ARABIDOPSIS H2A.Z INO80 PIF4 thermomorphogenesis transcription elongation 

分 类 号：Q94[生物学—植物学]