从水稻再生的研究进展看籼稻遗传转化的未来  

The future of genetic transformation of indica rice:perspectives from the research progress of rice regeneration

作　　者：王诗雨 蒋莹莹 徐恒[2] 朱英[2] 张华[2] WANG Shiyu;JIANG Yingying;XU Heng;ZHU Ying;ZHANG Hua(College of Chemistry and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;State Key Laboratory for Managing Biotic and Chemical Treats to the Quality and Safety of Agro-products,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江省农业科学院病毒学与生物技术研究所,农产品质量安全危害因子与风险防控省部共建国家重点实验室,杭州310021

出　　处：《植物生理学报》2021年第11期2069-2076,共8页Plant Physiology Journal

基　　金：国家自然科学基金(31871226)。

摘　　要：高效遗传转化体系的建立对于植物基因功能解析和分子育种利用均具有重要意义。目前虽已在水稻中建立了高效、稳定的转化体系,但仍受品种的限制。大部分籼稻的遗传转化难度较大,效率偏低。近年来,虽有一些提高籼稻遗传转化效率的方法被研发报道,但由于其中作用机制不明,技术优化困难重重,也未能被广泛推广应用。本文将对影响水稻遗传转化的因素、遗传基础和分子机制进行梳理和总结,期望能为未来籼稻高效遗传转化体系的建立提供一定的帮助。The establishment of efficient genetic transformation system in crop is quite important for the analysis of gene function and the utilization of molecular breeding.Although an efficient and stable transformation system has been established in rice,it is still limited by varieties.Genetic transformation of most indica rice is difficult and inefficient.In recent years,although some methods to improve the genetic transformation efficiency of indica rice have been developed and reported,it is still difficult to develop an optimized and wildly applicable system in most of indica varieties,as the underlying mechanism is unknown.In this paper,the regulatory factors,genetic basis and molecular mechanisms that affect genetic transformation in rice were reviewed and summarized.We hope it will be beneficial to the establishment of efficient genetic transformation system in indica rice in the future.

关 键 词：籼稻 遗传转化 植物组织培养 愈伤组织分化 分子机制 

分 类 号：S511.21[农业科学—作物学]