三种不同颜色藜麦籽粒中氨基酸及其衍生物的代谢组学分析  

Metabonomic analysis of amino acids and derivatives in three types of quinoa grains with different colors

作　　者：钱广涛 李祥羽[2] 周静雯 张蘅 李立新[1] QIAN Guangtao;LI Xiangyu;ZHOU Jingwen;ZHANG Heng;LI Lixin(Key Laboratory of Saline-alkali Vegetation Ecology Restoration,Ministry of Education,College of Life Sciences,Northeast Forestry University,Harbin 150040,China;Institute of Crop Resources,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;Shanghai Center for Plant Stress Biology,Chinese Academy of Sciences,Shanghai 201602,China)

机构地区：[1]东北盐碱植被恢复与重建教育部重点实验室,东北林业大学生命科学学院,哈尔滨150040 [2]黑龙江省农业科学院作物资源研究所,哈尔滨150086 [3]中国科学院上海植物逆境生物学研究中心,上海201602

出　　处：《植物生理学报》2021年第11期2192-2202,共11页Plant Physiology Journal

基　　金：国家自然科学基金(31570246);中央高校基本科研业务费专项资金(2572019CT03);黑龙江省科技计划省院合作项目(YS19B05);农业科技创新跨越工程杂粮杂豆科技创新专项(HNK2019CX05-5)。

摘　　要：为了了解不同颜色藜麦籽粒中氨基酸及其衍生物的组成成分和差异情况,以黑色、白色和红色藜麦籽粒为材料,利用超高效液相色谱串联质谱(UPLC-ESI-MS/MS)检测平台进行了广泛靶向代谢组学分析。三种藜麦籽粒中共检测到71种氨基酸类化合物,其中,黑-红比较组(黑vs红组)发现有28种、黑vs白组有19种、红vs白组有45种差异氨基酸类化合物。同时,黑vs红与红vs白之间有25种、黑vs白和红vs白之间有17种、黑vs红和黑vs白之间有9种共同差异氨基酸类化合物。KEGG注释及富集分析发现,不同颜色藜麦籽粒之间差异氨基酸类化合物参与的代谢通路中,氨基酸的生物合成、次生代谢产物的生物合成和氨酰tRNA的生物合成之间的差异较大。本研究结果对藜麦籽粒的营养价值评价以及优良品种选育具有重要意义。In order to understand the composition and differences of amino acids and their derivatives among the quinoa grains with different colors,black,white and red quinoa grains were used to perform the extensive targeted metabolomic analysis using the ultra-performance liquid chromatography tandem mass spectrometry(UPLC-ESI-MS/MS) platform.A total of 71 amino acids and their derivatives were detected in the three types of quinoa grains.Among these,28 differentially accumulated amino acids and their derivatives were figured out in the black-red comparison group(Black vs Red Group),did 19 in the Black vs White group and 45 in the Red vs White group.At the same time,25 co-differentially accumulated amino acids and their derivatives were detected between Black vs Red and Red vs White,did 17 between Black vs White and Red vs White,and 9 between Black vs Red and Black vs White groups.KEGG annotation and enrichment analysis indicated that in the metabolic pathways,the biosynthesis of amino acids,biosynthesis of secondary metabolites and biosynthesis of aminoacyl tRNA,in which the differentially accumulated amino acids involved,exhibited greater differences among the different colored grains.The results of this study are of great significance to the evaluation of the nutritional value of quinoa grains and the breeding of fine varieties.

关 键 词：藜麦 不同颜色籽粒 氨基酸及衍生物 代谢组学 

分 类 号：S519[农业科学—作物学]