复方大黄素黄芩素及其拆方对大鼠重症急性胰腺炎的治疗作用  

Effect of compound Emodin-Baicalein and its separated recipes on rat severe acute pancreatitis

作　　者：周蕊[1,2] 张健 黄娜[1] 陈海燕[1] 谭婷[1] 朱建宏[1] 张爱军[1] 杨军[1] 李君[1] 李宗芳[1,2] ZHOU Rui;ZHANG Jian;HUANG Na;CHEN Haiyan;TAN Ting;ZHU Jianhong;ZHANG Aijun;YANG Jun;LI Jun;LI Zongfang(National Local Joint Engineering Research Center of Biodiagnostics and Biotherapy, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China;Cadres Ward of General Surgery, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China)

机构地区：[1]西安交通大学第二附属医院生物诊断治疗国家地方联合工程研究中心 [2]安交通大学第二附属医院干部病房普通外科,陕西西安710004

出　　处：《西部医学》2021年第12期1727-1731,共5页Medical Journal of West China

基　　金：国家自然科学基金(81973659);陕西省重点研发计划(2021SF-38)。

摘　　要：目的探讨复方大黄素黄芩素组方的合理性,分析方中二组分对全方治疗重症急性胰腺炎(SAP)作用的影响。方法SPF级雄性SD大鼠120只,采用5%牛磺胆酸钠逆行胰胆管注射法制备大鼠重症急性胰腺炎模型,根据拆方分析实验设计,设正常对照组、假手术组、单纯SAP组、阳性对照药单倍剂量大黄素组(A组,大黄素3.2 mg/kg)、阳性对照药2倍剂量大黄素组(2A组,大黄素6.4 mg/kg)、单倍剂量黄芩素组(B组,黄芩素7 mg/kg)、2倍剂量黄芩素组(2B组,黄芩素14 mg/kg)、复方低剂量组(1/2AB组,大黄素1.6 mg/kg+黄芩素3.5 mg/kg)、复方中剂量组(AB组,大黄素3.2 mg/kg+黄芩素7 mg/kg)、复方高剂量组(2AB组,大黄素6.4 mg/kg+黄芩素14 mg/kg)进行实验,各组均12只大鼠。各组干预药物均于模型复制后立即依次尾静脉注射不同剂量药物溶液(2 mL/kg),正常对照组、假手术组、单纯SAP组尾静脉注射等量生理盐水。各组大鼠分别于给药后12 h全部处死,收集胰腺组织、血清,分别进行胰腺组织病理学评分、血清ɑ-淀粉酶活性测定。结果胰腺组织病理学评分和血清α-淀粉酶活性,各治疗组均明显低于单纯SAP组(P<0.05),AB组均明显低于2A和2B组(P<0.05)。各复方组(1/2AB、AB、2AB组)随着剂量增加,胰腺组织病理学评分降低。结论复方大黄素黄芩素的疗效好于2倍剂量大黄素,也好于2倍剂量黄芩素,组方中大黄素、黄芩素之间为协同作用,复方大黄素黄芩素组方合理。Objective To investigate the composition rationality of compound Emodin-Baicalein,and analyze the effects of two components in the treatment of severe acute pancreatitis(SAP).Methods The rat models of SAP were induced by retrograde injection of 5%sodium taurocholate into the common biliopancreatic duct.According to the experimental design of the regularity of recipe composition,male Sprague Dawley rats were randomly divided into the control group,Sham operation group,SAP group,A group(emodin,3.2mg/kg),2A group(emodin,6.4mg/kg),B group(baicalein,7mg/kg),2B group(baicalein,14mg/kg),1/2AB group(emodin 1.6mg/kg+baicalein 3.5mg/kg),AB group(emodin 3.2mg/kg+baicalein 7mg/kg)and 2AB group(emodin 6.4mg/kg+baicalein 14mg/kg).All the rats were sacrificed at 12 hours after model preparation.The histopathology score of pancreas and serum amylase activity were determined 12 hours after the surgery to evaluate the drug efficacy.Results Compared with the SAP group,pancreatic histopathological score and serumα-amylase activity decreased significantly in three treatment groups.Compared with groups 2A and 2B,the pancreatic histopathological score and serumα-amylase activity in group AB were significantly decreased.The pancreatic histopathological score in 1/2AB,AB and 2AB groups decreased with the dose increase.Conclusion The effect of AB group was better than that of 2A and 2B groups.There was a synergistic effect between emodin and baicalein in the Compound Emodin-Baicalein.The composition of Compound Emodin-Baicalein was reasonable and has the effect of treating SAP in rats.

关 键 词：大黄素 黄芩素 重症急性胰腺炎 相互作用 协同 

分 类 号：R657.51[医药卫生—外科学]