原发免疫性血小板减少症激素敏感与抵抗者血清蛋白质组学的研究  

Study of the serum proteomics between glucocorticoid-sensitive and glucocorticoid-resistant in primary immune thrombocytopenia

作　　者：张扬[1] 白菊[1] 张王刚[1] 何爱丽[1] ZHANG Yang;BAI Ju;ZHANG Wanggang;HE Aili(Department of Hematology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710004, China)

机构地区：[1]西安交通大学第二附属医院血液科,陕西西安710004

出　　处：《西部医学》2021年第12期1802-1806,共5页Medical Journal of West China

摘　　要：目的探讨原发免疫性血小板减少症(ITP)糖皮质激素治疗敏感与治疗抵抗患者血清蛋白质组学的差异。方法选择2016年3月~2017年3月我院住院ITP患者60例,其中激素敏感组32例,激素抵抗组28例。收集治疗前和随访4周后血清,利用弱阳离子磁珠联合基质辅助激光解吸电离飞行时间质谱技术(MALDI-TOF-MS)方法,寻找差异蛋白。结果两组患者治疗前血清蛋白指纹图谱相比较,在分子量700~10000 Da范围内共得到41个差异蛋白表达峰,但差异均无统计学意义(均P>0.05)。敏感组患者激素治疗前后比较,在分子量700~10000 Da范围内有5个差异蛋白(P<0.05),激素治疗后表达上调的蛋白有4个,相对分子量分别为9289.84、4644.96、7764.95和4964.12 Da,下调的蛋白有1个,分子量为6666.94 Da。激素抵抗患者激素治疗前后比较,在分子量700~10000 Da范围内共得到56个差异蛋白质表达峰,差异无统计学意义(P>0.05)。结论激素敏感者治疗前后存在5个差异表达的蛋白,可能与ITP的激素治疗效果密切相关。Objective To analyze the discrepancies in serum protein fingerprint between glucocorticoid(GC)-sensitive and GC-resistant individuals with primary immune thrombocytopenia(ITP).Methods After a 4-week GC treatment,patients with ITP were dived into the two groups.32 patients were assigned to the GC-sensitive group,and 28 patients were assigned to the GC-resistant group.Clinical parameters were compared between the two groups,the protein expression patterns from all specimens were determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MADLI-TOF-MS).Results The detection of Clinprot system and the analysis of CliprotoolsTM2.2 software shew that about 41 protein peaks were detected within the range of 700 Da and 10000 Da of molecular weight in the protein spectrum of serum specimen from 32 GC-sensitive patients and 28 GC-resistance patients,but there aren’t statistically differential(P>0.05).Five proteins with molecular weights of 9289.84,4644.96,6666.94,4964.12,and 7764.95 Da were differentially expressed after GC treatment in GC-sensitive group.And there aren't statistically differential after GC treatment in GC-resistance group.Conclusion We found five proteins in GC-sensitive group.These differential proteins may be closely related to the pathogenesis of ITP and the effect of the GC treatment.

关 键 词：原发免疫性血小板减少症 弱阳离子交换纳米磁珠 基质辅助激光解吸电离飞行时间质谱 血清蛋白质组学 

分 类 号：R558.2[医药卫生—血液循环系统疾病]