Multifunctional polyethyleneimine for synthesis of core-shell nanostructures and electrochemiluminescent detection of three AMI biomarkers  

作　　者：Xu Liang Lan Mi Zhengyou Yu Minghan Wang Yuecong Hu Xinhe Zheng Yi Shao Zhiwei Zhu Yuanhua Shao 

机构地区：[1]Beijing National Laboratory for Molecular Sciences,College of Chemistry and Molecular Engineering,Peking University,Beijing,100871,China

出　　处：《Science China Chemistry》2021年第12期2230-2238,共9页中国科学（化学英文版）

基　　金：supported by the National Key Research and Development Program of China(2016YFA0201300);the National Natural Science Foundation of China(22034001);Beijing National Laboratory for Molecular Sciences(BNLMS-CXXM-202008)。

摘　　要：Simplicity and stability of an analytical method are the key to practical applications.In order to achieve such a goal,a molecule with multifunction could be one of the strategies.In this work,the multifunctional characteristics of branched polyethylenemine(bPEI)were employed for verification of this hypothesis.Three types of amines in the bPEI can be used to synthesize core-shell nanostructures(bPEI@Au and SiO_(2)@bPEI@Au)in a simple one-pot process within 1 h,and as coreactants with over 500-fold enhancement for electrochemiluminescent(ECL)detection.Based on the remarkable performance of bPEI,a label-free ECL immunosensor and its array with the same assembly strategy were constructed for the simple and rapid determination of three acute myocardial infarction(AMI)biomarkers:cardiac troponin I(cTnI),human-type fatty acid binding protein(hFABP)and copeptin.Both methods exhibited excellent stability and sensitivity with wide linear range and low limits of detection down to fg/mL level by the photomultiplier tube and pg/mL level by the electron-multiplying charge-coupled device.

关 键 词：SIMPLICITY MULTIFUNCTION branched polyethylenemine ELECTROCHEMILUMINESCENCE AMI biomarkers 

分 类 号：R446[医药卫生—诊断学] O657.1[医药卫生—临床医学] TB383.1[理学—分析化学]