单载体吸附微生物的研究  

Study on Single Carrier Adsorption of Microorganisms

作　　者：王格 杨萌 杨明月 鲁琳[1] WANG Ge;YANG Meng;YANG Mingyue;LU Lin(School of Animal Science and Technology,Beijing Agricultural University,Beijing 101300;North China Key Laboratory of Urban Agriculture,Ministry of Agriculture and Rural Areas,Beijing 102206)

机构地区：[1]北京农学院动物科学技术学院,北京101300 [2]农业农村部华北都市农业重点实验室,北京102206

出　　处：《中国乳业》2021年第11期112-117,共6页China Dairy

基　　金：北京市奶牛产业创新团队项目(BAIC06-2021)。

摘　　要：微生物菌剂的保存一直是个难题,直接影响到微生物菌剂的应用。由于液态菌剂保藏效果不理想且使用较为不便,因此,找到一种吸菌量大,并能增加细胞密度和保持菌种存活力的载体显得尤为迫切。为研究出适合吸附微生物的单载体,进行了单载体吸附微生物培养并定期测定其纤维素酶酶活和过氧化氢酶酶活。通过数据分析,选出几种表现较好的载体,也为复合载体试验提供了筛选依据。研究表明,在过氧化氢酶酶活的测定中,麦麸及干牛粪的表现均比较优秀且3种菌都在第6~8天的时候出现峰值。在纤维素酶酶活的测定中,亦是麦麸与干牛粪的表现较为突出,而在后期接到麦麸上的3种菌的纤维素酶酶活都大幅度提高。麦麸、硅藻土、干牛粪及锯末这4种载体适合ZX5、ZZ9、ZH9三种微生物的吸附。The preservation of microbial agents has always been a difficult problem,which directly affects the application of microbial agents.Because the preservation effect of liquid bacteria is not ideal and the use is inconvenient,it is particularly urgent to find a carrier with large amount of bacteria,which can increase cell density and maintain the viability of bacteria.In order to develop a single carrier suitable for adsorbing microorganisms,the single carrier adsorbed microorganisms were cultured and their cellulase and catalase activities were measured regularly.Through the above data and analysis,several carriers with good performance are selected,which also provides data and screening for the composite carrier test.The results showed that the performance of wheat bran and dry cow dung were excellent in the determination of catalase activity,and the three bacteria peaked from the sixth to the eighth day.In the determination of cellulase activity,the performance of wheat bran and dry cow dung was more prominent,and the cellulase activities of the three bacteria received from wheat bran increased significantly in the later stage.Wheat bran,diatomite,dried cow dung and sawdust are suitable for the adsorption of ZX5,ZZ9 and ZH9 microorganisms.

关 键 词：单载体 吸附 微生物 

分 类 号：Q93-33[生物学—微生物学]