脊髓背角神经元STAT3/SDF-1信号通路在大鼠术后慢性疼痛形成中的作用  

Mechanism of spinal dorsal horn SDF-1 signaling pathway involved in chronic postsurgical pain

作　　者：孙扬 张璎 张海良[2] 李静[3] 孙文艳[4] SUN Yang;ZHANG Ying;ZHANG Hailiang;LI Jing;SUN Wenyan(Department of Rehabilitation Medicine,the Second Affiliated Hospital of Xi′an Medical University,Xi′an 710038,China;不详)

机构地区：[1]西安医学院第二附属医院康复医学科,西安710038 [2]西安医学院第二附属医院医务处,西安710038 [3]西安医学院第二附属医院院办,西安710038 [4]西安医学院第二附属医院耳鼻喉科,西安710038

出　　处：《实用医学杂志》2021年第22期2845-2850,共6页The Journal of Practical Medicine

基　　金：陕西省科技厅自然科学基础研究计划项目(编号:2018JM7052);陕西省教育厅专项科研计划项目(编号:18JK0675)。

摘　　要：目的本文旨在探讨脊髓背角神经元信号转导和转录激活因子3(STAT3)/基质细胞衍生因子1(SDF-1)信号通路在大鼠形成术后慢性疼痛中的作用与机制。方法建立大鼠皮肤肌肉切口牵拉(SMIR)术后慢性疼痛模型,鞘内用或不用SDF-1中和性抗体antiSDF-1与STAT3抑制剂S3I-201,观察术前及术后不同时点手术同侧机械性撤足阈值,脊髓背角SDF-1和转录激活因子3(STAT3)表达水平及细胞定位。另外,染色质免疫共沉淀(ChIP)检测STAT3与SDF-1启动子的相互作用。结果与术前相比,术后第5、10、20天大鼠机械性撤足反射阈值显著降低,伴有脊髓背角神经元SDF-1和p-STAT3表达上调,且均与Neun阳性神经元共染。antiSDF-1和S3I-201预处理均可缓解SMIR术后机械性撤足阈值改变,S3I-201还降低术后SDF-1转录和翻译的上调。SMIR术后增加脊髓背角STAT3与SDF-1启动子结合。结论 SMIR可能通过增强脊髓背角STAT3向SDF-1基因启动子的募集,上调SDF-1的表达,从而促进了机械性痛觉过敏的发生发展。Objective To investigate the effect and mechanism of STAT3/SDF-1 signaling pathway of spinal dorsal horn neurons in postoperative chronic pain. Methods A SMIR animal model,with or without antiSDF-1 and S3I-201 intrathecally,was established. Mechanical withdrawal threshold on the ipsilateral side before and after operation,expression level of SDF-1 and STAT3,and cell location in the spinal dorsal horn were observed. In addition,ChIP was used to detect the interaction between STAT3 and SDF-1 promoter. Results The mechanical withdrawal threshold of rats on the 5 th,10 th,and 20 th day after operation was significantly reduced,accompanied by up-regulation of the expression of SDF-1 and p-STAT3 in the spinal dorsal horn neurons,and all co-stained with Neun-positive neurons. AntiSDF-1 and S3I-201 pretreatment alleviated the changes in the mechanical withdrawal threshold after SMIR,and S3I-201 also reduced the upregulation of SDF-1 transcription and translation after surgery. After SMIR,the combination of STAT3 and SDF-1 promoter in the dorsal horn of the spinal cord was increased. Conclusions STAT3/SDF-1 signaling pathway of spinal dorsal horn neurons is involved in the formation of postsurgical chronic pain.

关 键 词：皮肤肌肉切口牵拉 基质细胞衍生因子1 信号转导和转录激活因子3 术后慢性疼痛 

分 类 号：R338[医药卫生—人体生理学]