非小细胞肺癌中高表达piR-1366的临床病理意义及对肿瘤转移的影响  

Clinicopathological significance of piR-1366 overexpression in NSCLC and its effect on tumor metastasis

作　　者：郭虹 韩微 马怡君 周彩婷 冯康 王磊[4] 史宏灿[1] GUO Hong;HAN Wei;MA Yijun;ZHOU Caiting;FENG Kang;WANG Lei;SHI Hongcan(Medical College of Yangzhou University,Yangzhou 225009,China;Department of Cardiothoracic Surgery,Affiliated Hospital of Yangzhou University,Yangzhou 225009,China;不详)

机构地区：[1]江苏省扬州大学医学院,江苏扬州225009 [2]大连医科大学,辽宁大连116044 [3]江苏省扬州大学附属医院心胸外科,江苏扬州225009 [4]江苏省扬州大学附属医院病理科,江苏扬州225009

出　　处：《实用医学杂志》2021年第21期2739-2742,2747,共5页The Journal of Practical Medicine

基　　金：国家卫生健康委医药卫生科技发展研究中心(编号:WA2021RW27);江苏省扬州市科技局国际科技合作项目(编号:YZ2020200)。

摘　　要：目的探讨piRNA-1366(piR-1366)在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达、临床病理意义及对肿瘤转移的影响.方法用piRNA基因芯片对NSCLC piRNAs表达谱进行分析,采用qRT-PCR证实piR-1366在NSCLC的表达;采用RNA原位杂交技术(RISH)检测87例NSCLC组织中piR-1366的表达,分析piR-1366表达与患者临床病理参数的关系;采用Transwell小室检测piR-1366对肺癌细胞迁移的影响,采用裸鼠肺转移模型检测piR-1366对肺癌细胞远处转移的影响.结果 piRNAs表达谱和qRT-PCR均检测出piR-1366在NSCLC中表达水平升高,RISH显示piR-1366主要为阳性表达.piR-1366高表达组与低表达组NSCLC淋巴结转移差异有统计学意义(P <0.05),但年龄、性别、肿瘤部位、肿瘤大小、肿瘤分级及TNM分期差异无统计学意义(均P> 0.05).Transwell实验结果显示过表达piR-1366促进了A549和H1650的迁移,而沉默表达则阻碍了肺癌细胞的迁移(P <0.05).在裸鼠肺转移模型中沉默piR-1366表达组(si-piR-1366)肺转移癌结节要比对照组少(P <0.05).结论 piR-1366肺癌细胞的转移有促进作用,可为研究piR-1366治疗转移性NSCLC提供靶向依据.Objective To investigate the expression and clinicopathological significance of piR-1366 in non-small cell lung cancer(NSCLC)and its effect on tumor metastasis. Methods piRNA microarray was used to analyze the expression profile of piRNAs in NSCLC. The expression of piR-1366 in NSCLC was confirmed again by qRT-PCR;The expression of piR-1366 in 87 cases of NSCLC was detected by RNA in situ hybridization(RISH),and the correlation was analyzed between the expression of piR-1366 and clinicopathological parameters of patients. Transwell chamber was used to detect the effect of piR-1366 on the migration of lung cancer cells,and lung metastasis model of nude mouse was used to detect the effect of piR-1366 on the metastasis. Results The expression level of piR-1366 were increased in NSCLC by piRNAs expression profile and qRT-PCR,and the positive expression of piR-1366 was showed in FISH. There were statistically significant differences in lymph node metastasis between pir-1366 high-expression group and low-expression group(P<0.05),but there were no statistically significant differences in age,gender,tumor site,tumor size,tumor grade and TNM stage(all P>0.05). Transwell results showed that over-expression of PIR-1366 promoted the migration of A549 and H1650,while silencing of PIR-1366 inhibited the migration of LUNG cancer cells(P < 0.05). In the nude mouse model of lung metastasis,there were fewer nodules of lung metastasis in the silenced PIR-1366 group(SI-PIR-1366)than in the control group(P<0.05). Conclusion piR-1366 could promote the metastasis of lung cancer cells,and provide targeted basis for the study of piR-1366 in the treatment of metastatic NSCLC.

关 键 词：piR-1366 非小细胞肺癌 临床病理意义 迁移 转移 

分 类 号：R734.2[医药卫生—肿瘤]