猪脾转移因子提高LaSota株鸡新城疫弱毒疫苗的免疫保护率  

Improvement of the Immune Protection Rate on Newcastle Disease Virus Attenuated Vaccine LaSota Strain by Pig Spleen Transfer Factor

作　　者：徐磊[1] 钟佳莲 余勋信[1] 刘毅发 黄瑜 刘小龙[4] 赖隆永 闫丽萍 许秀梅 宋素泉 张渊魁[6] XU Lei;ZHONG Jialian;YU Xunxin;LIU Yifa;HUANG Yu;LIU Xiaolong;LAI Longyong;YAN Liping;XU Xiumei;SONG Suquan;ZHANG Yuankui(Fujian Agricultural Vocational Technical College,Fuzhou 350119,China;Fujian Provincial Key Laboratory for Avian Diseases Control and Prevention,Fuzhou 350013,China;Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou 350013,China;Present(Fuzhou)Biotech Company Limited,Fuzhou 350500,China;College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China;Zhaofenghua Biotechnology(Nanjing)Co.,Ltd.,Nanjing 211102,China)

机构地区：[1]福建农业职业技术学院,福州350119 [2]福建省禽病防治重点实验室,福州350013 [3]福建省农业科学院畜牧兽医研究所,福州350013 [4]派生特(福州)生物科技有限公司,福州350500 [5]南京农业大学动物医学院,南京210095 [6]兆丰华生物科技(南京)有限公司,南京211102

出　　处：《畜牧兽医学报》2021年第12期3578-3587,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：福建省第七批引才“百人计划”;福建省科技计划项目(2019 N0025);江苏省博士后科研资助计划(2019K072);福建省高层次人才和青年优秀人才访学研修资助计划(闽人社文〔2019〕239号);福州市科技计划项目(2020-PT-149);福建省畜禽疫病防控技术重大研发平台项目(2014 N2003);福建省禽病防治重点实验室开放基金课题(KF201902);福建省职业院校专业带头人培养计划(闽教办师〔2019〕40号);福建省科技计划项目(2021S2003)。

摘　　要：旨在了解猪脾转移因子(TF)对新城疫病毒(NDV)弱毒疫苗LaSota株的免疫增强效果及机制。本研究分别采用不同剂量(10^(-2)、10^(-3)、10^(-4)、10^(-5)羽份)LaSota疫苗株单独(单独免疫组)、LaSota疫苗株与TF联合(联合免疫组)免疫SPF鸡,14 d后以NDV F 48 E 9强毒攻毒,同时设立对照组(非免疫攻毒)和空白组(非免疫非攻毒),并采用ELISA方法与蛋白质芯片技术分别测定外周血IL-4、IFN-γ与IL-12 P40浓度。结果显示,鸡免疫10^(-2)、10^(-3)、10^(-4)、10^(-5)羽份时疫苗攻毒保护率和半数保护量(PD_(50))如下:单独免疫组分别为100%、55%、0%、0%和0.0008羽份,联合免疫组分别为100%、75%、0%、0%和0.0005羽份,对照组和空白组死亡率分别为100%和0%。免疫10^(-3)羽份疫苗后,联合免疫组IL-4和IFN-γ含量均高于其他组,并于第7、14天差异极显著(P<0.01);攻毒后,联合免疫组IL-4、IFN-γ和IL-12 P40含量均高于其他组,IL-4于第1、14天,IFN-γ于第1、3天,IL-12 P40于第1天差异极显著(P<0.01)。综上表明,TF可增强IFN-γ、IL-12介导的细胞免疫和IL-4介导的体液免疫,提高LaSota株弱毒疫苗的免疫保护率,降低疫苗PD_(50);在抵抗NDV F 48 E 9强毒攻击时,联合免疫组的免疫保护率明显高于单独免疫组。This study was conducted to evaluate the immune enhancement effect and mechanism of pig spleen transfer factor(TF)on Newcastle disease virus(NDV)attenuated vaccine LaSota strain.Specific pathogen free(SPF)chickens were inoculated with different doses(10^(-2),10^(-3),10^(-4),10^(-5))of NDV strain LaSota(vaccination group without TF),NDV strain LaSota combined with TF(vaccination group with TF),respectively,and challenged with virulent NDV strain F 48 E 9 on 14 days post vaccination(dpv).The control group(non-immunized but challenged)and blank group(non-immunized and non-challenged)were set up simultaneously.In addition,the change of IL-4,IFN-γand IL-12 P40 in peripheral blood were analyzed by ELISA and protein chip technology.The protection rate of different doses and median protective dose(PD_(50))were 100%,55%,0%,0%,0.0008 doses in the plume in vaccination group without TF,respectively,and 100%,75%,0%,0%,0.0005 doses in the plume in vaccination group with TF.In contrast,the mortality of control group and blank group were 100%and 0%.After immunization with 10^(-3)dose of vaccine,the levels of IL-4 and IFN-γin vaccination group with TF were higher than in other groups and the difference was extremely significant on 7 and 14 dpv(P<0.01).After challenge,the levels of IL-4,IFN-γand IL-12 P40 in vaccination group with TF were higher than in other groups.The differences of IL-4 on 1 and 14 days post challenge(dpc),IFN-γon 1 and 3 dpc,IL-12 P40 on 1 dpc were extremely significant(P<0.01).These findings indicated that TF could reinforce cellular immunity by IFN-γ,IL-12 and humoral immunity by IL-4,improve the immune protection rate of NDV attenuated vaccine LaSota strain and reduce the PD_(50).Vaccination group with TF was more effective than vaccination group without TF against virulent NDV strain F 48 E 9.

关 键 词：猪脾转移因子 鸡新城疫弱毒LaSota株 鸡新城疫强毒F_(48)E_(9)株 免疫保护率 细胞因子 

分 类 号：S858.315.3[农业科学—临床兽医学]