内生大豆间座壳Diaporthe sojae的子囊壳促生培养及鉴定  

Perithecia of Promoting Culture and Identification of Endophytic Diaporthe sojae

作　　者：豆明珠 蒲顺昌 闫淑珍[1] 陈双林[1] Dou Mingzhu;Pu Shunchang;Yan Shuzheng;Chen Shuanglin(School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)

机构地区：[1]南京师范大学生命科学学院,江苏南京210023

出　　处：《南京师大学报（自然科学版）》2021年第4期60-68,共9页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：国家科技基础性工作专项(2013FY110400).

摘　　要：本文介绍了对野大豆内生间座壳真菌的子囊壳促生培养方法,结合形态特征和分子序列对分离和培养的野大豆内生间座壳真菌进行了鉴定.采用马铃薯葡萄糖琼脂培养基(PDA)和水琼脂培养基(WA),接种分离获得的野大豆内生间座壳菌株DYYP4342菌饼,同时分别在接种菌饼两侧平行放置灭菌大豆茎秆和灭菌松针,以诱导野大豆内生间座壳Diaporthe sojae在大豆茎秆和灭菌松针上产生子囊壳,之后在25℃、12 h光照与12 h黑暗交替处理下培养,添加灭菌大豆茎秆和灭菌松针的培养基中持续产生子囊壳,数量显著多于对照组马铃薯葡萄糖琼脂培养基(PDA)和水琼脂培养基(WA).通过形态特征和rDNA-ITS分子序列鉴定该分离菌株为大豆间座壳Diaporthe sojae,该菌此前被报道发生在栽培大豆上,引起茎荚枯病,作为内生真菌在野大豆上则为首次记录,文中比较了野大豆内生Diaporthe sojae与栽培大豆上发生的Diaporthe sojae及其他Diaporthe种的差异.ITS序列系统发育分析结果支持Diaporthe kochmanii为Diaporthe sojae的命名异名的观点.In this paper,a method for increasing the quantity and promoting the maturity of perithecia of an endophytic Diaporthe species from Glycine soja is introduced and described.The morphological characteristics and molecular sequence of the isolates and cultures of endogenous mesial shell fungi from Glycine soja are identified.Potato dextrose agar(PDA)and water agar(WA)were used to inoculate the isolated Glycine soja endogenous mesocapsid strain cake,while sterilized soybean stalks and extinct bacteria were placed in parallel on both sides of the inoculated fungal strain pine needles,which are used to induce to produce perithecia on sterilized soybean stalks and sterilized pine needles,and then cultured at 12 h light vs.12 h dark and 25℃,perithecia were continuously produced in the medium with sterilized soybean stalks and sterilized pine needles,and the number was significantly greater than that in the medium of PDA.The isolate was identified as Diaporthe sojae by its morphological characteristics and molecular sequence.This species was previously reported to occur on Glycine max,causing stem blight,and it was the first record on Glycine soja as endophytic fungi.The results of phylogenetic analysis of ITS sequences support Diaporthe kochmanii is an idea of naming a different name for Diaporthe sojae.

关 键 词：野大豆 内生真菌 子囊壳 培养方法 

分 类 号：Q945[生物学—植物学]