顺9,反11-共轭亚油酸对奶牛乳腺上皮细胞炎症因子的影响  

Effects of Cis 9,trans 11-Conjugated Linoleic Acid on Inflammatory Factors in Bovine Mammary Epithelial Cells

作　　者：张航[1] 张腾龙[2] 王雨琼 魏曼琳[1] 牛化欣[1] ZHANG Hang;ZHANG Tenglong;WANG Yuqiong;WEI Manlin;NIU Huaxin(College of Animal Science and Technology,Inner Mongolia University for Nationalities,Inner Mongolia Tongliao 028000,China;College of Animal Science,Inner Mongolia Agricultural University,Inner Mongolia Hohhot 010018,China)

机构地区：[1]内蒙古民族大学动物科技学院,内蒙古通辽028000 [2]内蒙古农业大学动物科学学院,内蒙古呼和浩特010018

出　　处：《饲料工业》2021年第23期9-12,共4页Feed Industry

基　　金：内蒙古自治区自然科学基金[2018LH03011、2018LH03017、2019BS03023];内蒙古民族大学博士启动基金[KYQD18053]。

摘　　要：比较不同浓度顺9,反11-共轭亚油酸(c9,t11-CLA)的抗炎作用。以奶牛乳腺上皮细胞(BMECs)为模型,添加确定对细胞增殖无影响的浓度梯度的c9,t11-CLA,检测炎症因子TNF-α(肿瘤坏死因子-α)、IL-6(白细胞介素-6)、IL-8(白细胞介素-8)、IL-10(白细胞介素-10)含量及TNF-α、IL-6、IL-8的mRNA表达量的影响。与对照组相比,添加200μmol/L c9,t11-CLA显著降低BMECs中IL-8和TNF-α含量以及mRNA表达量(P<0.05),添加100μmol/L c9,t11-CLA显著增加TNF-α含量和IL-8的mRNA表达量(P<0.05)。试验结果表明,200μmol/L c9,t11-CLA对BMECs中促炎因子有抑制作用,可为后续c9,t11-CLA的抗炎机制研究奠定数据基础。This experiment was conducted to compare the effects of different concentrations of cis 9,trans 11-conjugated linoleic acid(c9,t11-CLA)on anti-inflammatory.Bovine mammary epithelial cells(BMECs)were used as the model.The c9,t11-CLA with different concentrations were added to determine the concentration gradient which had no effects on cell proliferation.The effects of the contents of inflammatory cytokines TNF-α,IL-6,IL-8 and IL-10 and mRNA expressions of TNF-α,IL-6 and IL-8 were detected.Compared with the control group,adding 200μmol/L c9,t11-CLA group significantly decreased the content and mRNA ex⁃pression of IL-8 and TNF-αin BMECs(P<0.05),adding 100μmol/L c9,t11-CLA group significantly increased TNF-αcontent and IL-8 mRNA expression(P<0.05).The experiment results showed that 200μmol/L c9,t11-CLA inhibit the pro-inflammatory factors in BMECs,which could lay a data founda⁃tion for the study on the anti-inflammatory mechanism of c9,t11-CLA.

关 键 词：奶牛 乳腺上皮细胞 顺9 反11-共轭亚油酸 细胞增殖 炎症因子 

分 类 号：S816.32[农业科学—饲料科学]