日本血吸虫p38MAPK的克隆、表达和功能初步分析  

Molecular Characterization and Functional Analysis of p38MAPK in Schistosoma japonicum

作　　者：李慧民 李雪 Bikash R.Giri 刘军涛[1] 夏天奇[1] 王立辉[1,2] 杜鹏飞 陈永军[1] Hanif Ullah Abdul Qadeer 程国锋[1] LI Huimin;LI Xue;Bikash R.Giri;LIU Juntao;XIA Tianqi;WANG Lihui;DU Pengfei;CHEN Yongjun;Hanif Ullah;Abdul Qadeer;CHENG Guofeng(Key Laboratory of Animal Parasitology of Ministry of Agriculture and Rural Affairs,Shanghai Veterinary Research Institute,CAAS,Shanghai 200241,China;Tianjin Agricultural University,Tianjin 300384,China)

机构地区：[1]中国农业科学院上海兽医研究所农业农村部动物寄生虫学重点实验室,上海200241 [2]天津农学院,天津300384

出　　处：《中国动物传染病学报》2021年第6期35-41,共7页Chinese Journal of Animal Infectious Diseases

基　　金：国家自然科学基金。

摘　　要：丝裂原相关蛋白激酶(MAPK)信号通路在血吸虫的发育和产卵过程中发挥重要的调节作用,已有研究表明p38MAPK可调控曼氏血吸虫的发育,并参与雌性成熟过程。为此,本研究对日本血吸虫p38MAPK(Sjp38MAPK)进行了克隆、表达,并对其功能进行了初步分析。结果表明:Sjp38MAPK长度1038 bp,预测分子量39.58 kDa;生物信息学分析表明Sjp38MAPK与曼氏血吸虫和埃及血吸虫的同源性分别为93%和90%;qRT-PCR分析表明Sjp38MAPK在成虫中的表达量较高于虫卵、尾蚴和童虫时期;进一步利用siRNA抑制Sjp38MAPK表达,发现虫体卵壳蛋白的合成,运动以及细胞活性等方面受到影响。总之,本研究初步表明了Sjp38MAPK在日本血吸虫的发育和产卵过程中可能同样发挥重要的调节作用,值得深入研究。Mitogen associated protein kinase (MAPK) signaling pathway plays an important regulatory role in the regulation of development and sexual maturation in many organisms.Previous studies have indicated the important regulatory role of p38MAPK in development of Schistosoma mansoni.In the present study,we characterized S.japonicum p38MAPK (Sjp38MAPK) and evaluated its role in worm motility and cell viability.Bioinformatic analysis revealed that Sjp38MAPK shared 93% and 90% sequence identities with S.mansoni and S.haematobium,respectively.Phylogenetic tree analysis showed evolutionary closeness to other schistosoma spp.qRT-PCR analysis indicated the relatively high expression in adults at the transcriptional levels as compared with that in eggs,cercariae and schistosomula.siRNA silencing of Sjp38MAPK could affect the expressions of eggshell protein genes and influence worm motility and cellular viability.Overall,our preliminary study suggested the important role of Sjp38MAPK in development and egg production inS.japonicum.

关 键 词：日本血吸虫 P38MAPK 蛋白激酶 发育 产卵 

分 类 号：S852.735[农业科学—基础兽医学]