鸭疫里默氏杆菌弱毒株的发酵培养  

Industrial Fermentation Technology of the Attenuated Strain of Riemerella anatipestis

作　　者：李林林 孙敏华 董嘉文 吴彩艳 廖申权 孙铭飞 吕敏娜 邝瑞欢 张建峰 徐志宏 LI Linlin;SUN Minhua;DONG Jiawen;WU Caiyan;LIAO Shenquan;SUN Mingfei;LYU Minna;KUANG Ruihuan;ZHANG Jianfeng;XU Zhihong(Institute of Animal Health,Guangdong Academy of Agriculture Sciences Key Laboratory of Livestock Disease Prevention and Treatment of Guangdong Province Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Technigues of Guangdong Province,Ministny of Agriculture,guangdong 510640,China;Maoming Branch,Guangdong Laboratory of Lingnan Modern Agriculture,Guangdong 525000,China)

机构地区：[1]广东省农业科学院动物卫生研究所广东省畜禽疫病防治研究重点实验室农业农村部兽药与诊断学科群广东科学观测实验站,广州510640 [2]岭南现代农业科学与技术广东省实验室茂名分中心,茂名525000

出　　处：《中国动物传染病学报》2021年第6期90-94,共5页Chinese Journal of Animal Infectious Diseases

基　　金：国家重点研发计划(2017YFD0500800);广东省自然科学基金温氏集团联合基金-重点项目(2018B030315002)。

摘　　要：1型鸭疫里默氏杆菌在华南地区流行,本试验在获得1型鸭疫里默氏杆菌天然弱毒株SG株的基础上,采用具有在线控制pH和溶解氧(DO)的5 L发酵罐,通过对发酵培养基、发酵培养过程中DO值、发酵培养转速进行优化,获得1型鸭疫里默氏杆菌弱毒株SG株。结果显示:SG株发酵培养的最适培养基配方中每升培养基含胰蛋白胨17.5 g、大豆蛋白胨7.5 g、酵母提取物7.5 g、葡萄糖2.5 g;SG株发酵培养的最适DO值为20%、最适转速为150 rpm,当发酵8 h活菌数达到高峰(1.27×10^(10)CFU/mL);以获得的优化工艺在GMP 生产车间规模化发酵生产 3 批鸭疫里默杆菌弱毒株SG株,收获弱毒菌液的活菌数均高于1.0×10^(10)CFU/mL。本试验为鸭疫里默氏杆菌弱毒疫苗抗原的高效制备提供了指导。Riemerella anatipestis type 1 is prevalent in South China.In this experiment,industrial propagation of the attenuated SG strain of R.anatipestis type 1 was developed and optimized using 5 L fermentation tank with on-line control of pH and dissolved oxygen (DO) regarding the liquid medium,DO value and fermentation speed in the fermentation process.The optimum fermentation medium per liter for SG strain contained 17.5 g tryptone,7.5 g soybean peptone,7.5 g yeast extract and 2.5 g glucose.The optimum DO value and rotational speed of SG strain fermentation were 20% and 150 rpm,respectively.The number of viable bacteria reached a peak(1.27 ×10^(10)CFU/mL) after 8 hours of fermentation.Three batches of the attenuated SG strain were produced in a GMP workshop for large-scale industrial fermentation and the viable bacteria of each batch was higher than 1.0×10^(10)CFU/mL at harvest.This experiment provided preliminary guidance for the efficient preparation of the attenuated vaccine antigen of R.anatipestis.

关 键 词：鸭疫里默氏杆菌 弱毒株 发酵培养 

分 类 号：S852.612[农业科学—基础兽医学]