禾谷镰刀菌中的新镰刀菌素  

New Fusarins from Fusarium graminearum

作　　者：林月婷 杨谦 王婉秋 李秋菊 汤志军 唐威华 陶疆[1] 刘文[2,4] Lin Yueting;Yang Qian;Wang Wanqiu;Li Qiuju;Tang Zhijun;Tang Weihua;Tao Jiang;Liu Wen(Department of General Dentistry,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University School of Medicine,College of Stomatology,Shanghai Jiao Tong University,National Center for Stomatology,National Clinical Research Center for Oral Diseases,Shanghai Key Laboratory of Stomatology,Shanghai 200011;State Key Laboratory of Bioorganic and Natural Products Chemistry,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Shanghai 200032;CAS Center for Excellence in Molecular Plant Sciences,Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai 200032;Huzhou Center of Bio-synthetic Innovation,Shanghai Institute of Organic Chemistry,Chinese Academy of Sciences,Huzhou,Zhejiang 313000)

机构地区：[1]上海交通大学医学院附属第九人民医院口腔综合科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学中心,上海市口腔医学重点实验室,上海200011 [2]中国科学院上海有机化学研究所,生命有机化学国家重点实验室,上海200032 [3]中国科学院植物生理生态研究所,分子植物科学创新中心,上海200032 [4]中国科学院上海有机化学研究所,湖州生物制造中心,浙江湖州313000

出　　处：《有机化学》2021年第11期4504-4509,共6页Chinese Journal of Organic Chemistry

基　　金：国家自然科学基金(Nos.81974495,32030002,21977109,21621002);科技部重大专项(Nos.2018YFA090100,2019YFA0905400)资助项目.

摘　　要：镰刀菌素是由植物病原真菌产生的一类聚酮-非核糖体肽真菌毒素.其核心骨架的构筑依赖于一个不寻常的聚酮-非核糖体肽杂合酶.报道了禾谷镰刀菌中两个不同类型的新镰刀菌素,镰刀菌素X1(1)和镰刀菌素Y(2).其中,化合物1为聚酮-非核糖体肽杂合化合物,化合物2属于聚酮类化合物.通过基因敲除、高分辨质谱分析证实它们是由同一个生物合成基因簇产生的主线产物和旁支产物.该结果为后期进一步研究镰刀菌素生物合成提供了研究基础,也进一步为该病原真菌侵染禾谷类作物的机制研究提供了化学多样性.Fusarins as a group of polyketide-nonribosomal peptide hybrid mycotoxins,are produced by the plant pathogenic fungus Fusarium.The building of core framework depends on an unusual iterative type I polyketide synthase(PKS)fused to a non-ribosomal peptide synthetase(NRPS).Two new compounds,fusarins X1(1)and Y(2),were isolated from Fusarium graminearum.Fusarin X1(1)possesses a PKS-NRPS hybrid framework,and fusarin Y(2)is a polyketide.It is demonstrate that they are the main and shunt products in fusarins biosynthetic pathway,respectively,by gene inactivation and HRESIMS analyses.These findings not only lay the foundation for understanding of the fusarins biosynthetic logic,but also provide chemical diversity to further explore the concerning infect mechanism.

关 键 词：禾谷镰刀菌 镰刀菌素 生物合成 基因敲除 

分 类 号：S435.121.45[农业科学—农业昆虫与害虫防治]