阿胶对皮肤屏障损伤的修复作用  

Repair effect of colla corii asini on the damaged skin barrier function

作　　者：樊雨梅 帖航 赵海晴 苏宁[2] 廖峰 FAN Yu-mei;TIE Hang;ZHAO Hai-qing;SU Ning;LIAO Feng(National Engineering Research Center for Gelatin-based Traditional Chinese Medicine,Dong-E-E-Jiao Co.Ltd.,Liaocheng,Shandong 252201,China;Chinese Academy of Inspection and Quarantine,Beijing 100176,China)

机构地区：[1]东阿阿胶股份有限公司国家胶类中药工程技术研究中心,山东聊城252201 [2]中国检验检疫科学研究院,北京100176

出　　处：《日用化学品科学》2021年第12期18-22,共5页Detergent & Cosmetics

基　　金：山东省泰山产业领军人才传统产业创新类项目(TSCY20170233)。

摘　　要：探讨阿胶在皮肤屏障损伤方面的作用。通过八肽胆囊收缩素(CCK-8)法测定细胞存活率,酶联免疫吸附检测方法(ELISA)法测定Ⅰ型胶原蛋白和IL-1α含量水平,划痕法测定细胞迁移率,采用四唑盐比色法(MTT)法测定屏障指数(ET_(50)),评价阿胶在皮肤屏障损伤修复方面的作用。结果表明,1%以下浓度的阿胶对成纤维细胞无毒性作用,且可显著促进成纤维细胞增殖。阿胶能够显著促进成纤维细胞的迁移和I型胶原蛋白的表达。5%阿胶能够显著修复受损皮肤屏障、抑制IL-1α的表达,效果与地塞米松类似。阿胶能够有效修复和缓解十二烷基硫酸钠造成的皮肤屏障损伤和刺激。The repair effect of colla corii asini on the damaged skin barrier function was studied.Cell viability was determined by CCK-8 method.The production of Col Ⅰ and IL-1α was measured by ELISA.The cell migration was determined by scratch method.The ET_(50) was determined by MTT.The results show that colla corii asini at the concentrations below 1% has no toxic effect on fibroblasts and can significantly promote the proliferation of fibroblasts.1% colla corii asini can significantly increase the cell migration rate of NHDFs,and the effect is similar to that of 2% fetal bovine serum.Colla corii asini at a concentration of 1% can significantly promote the expression of type I collagen in NHDFs,but the effect is weaker than that of ascorbic acid of 15μmol/L.5% colla corii asini can significantly repair the damaged skin barrier and inhibit the expression of IL-1α,which is similar to dexamethasone.Colla corii asini can effectively repair and relieve the skin barrier damage and irritation caused by sodium lauryl sulfate.

关 键 词：阿胶 成纤维细胞 三维表皮模型 皮肤屏障修复 

分 类 号：TQ658[化学工程—精细化工]