透明质酸钠舒敏抗炎修复效果研究  

Study on the soothing,anti-inflammatory and repairing effects of sodium hyaluronate

作　　者：朱永刚 张建勇 王兰芝[2] ZHU Yong-gang;ZHANG Jian-yong;WANG Lan-zhi(Shandong Topscience Biotech Co.,Ltd.,Rizhao,Shandong,276800,China;Qilu University of Technology,Jinan,Shandong 250000,China)

机构地区：[1]山东众山生物科技有限公司,山东日照276800 [2]齐鲁工业大学,山东济南250000

出　　处：《日用化学品科学》2021年第12期36-42,共7页Detergent & Cosmetics

摘　　要：为了研究透明质酸钠的舒敏、抗炎、修复功效,采用C48/80刺激肥大细胞模型,通过肥大细胞脱颗粒的形态学观察和脱颗粒率来评价其舒敏功效;选用UVB刺激角质形成细胞建立皮肤炎症损伤体外模型,通过检测炎症因子TNF-α、IL-1α表达情况来评价其抗炎功效;将透明质酸钠均匀涂布于实验构建的“SLS-Epikutis”皮肤刺激性损伤模型表面以模拟人体使用过程,通过检测模型组织形态学结构变化及屏障相关蛋白,评价其促进“SLS-Epikutis”刺激性损伤模型屏障修复的功效。结果表明,与1 mg/mL的色甘酸钠相比,7.81μg/mL样品舒敏效果较好;与0.01%的地塞米松相比,1 mg/mL样品抗炎性较好;与50μmol/L的WY14643相比,10 mg/mL的样品具有修复效果,由此说明一定浓度的透明质酸钠具有较好的舒敏、抗炎和修复功效。The soothing,anti-inflammatory and repairing effects of sodium hyaluronate were studied.The C48/80 mast cell stimulation model was used to evaluate the soothing effect of sodium hyaluronate through the observation of morphology and degranulation rate of mast cells.A model of skin inflammatory injury was established through stimulating keratinocytes by UVB in vitro.The antiinflammatory effect was evaluated by detecting the expression of inflammatory cytokines TNF-α and IL-1α.The skin irritation injury model"SLS-Epikutis"was established in which sodium hyaluronate was evenly coated on the surface of this model to simulate the use to human.The effect of sodium hyaluronate to promote the barrier repair of the irritation injury model“SLS-Epikutis”was evaluated by detecting the changes in the histomorphological structure of the model and the barrier related proteins.The results show that sodium hyaluronate has better soothing effect than 1 mg/mL sodium cromoglycate at the concentration of 7.81μg/mL;sodium hyaluronate has better anti-inflammatory activity than 0.01% dexamethasone at the concentration of 1 mg/mL;sodium hyaluronate has better repairing effect than 50μmol/L WY14643 at the concentration of 10 mg/mL.In summary,a certain concentration of sodium hyaluronate has better soothing,anti-inflammatory and repairing effects.

关 键 词：透明质酸钠 生物活性 抗炎 舒敏 修复 

分 类 号：TQ658[化学工程—精细化工]